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Zur Induktion von Schwesterchromatidaustausch durch Diethylstilböstrol und Mitomycin C bei Lymphocyten von schwangeren Frauen und deren Neugeborenen

  • Chen Xue-min
  • Schmidt B. 
  • Dunkelberg H. 
  • Borneff J. 
Article

Zusammenfassung

Lymphozyten von Müttern und ihren Neugeborenen wurden mit verschiedenen Konzentrationen von Diethylstilböstrol (10-6M, 10-5M und 10-4M) und Mitomycin C (0,003, 0,009 und 0,03 μg/ml) behandelt und zur Bestimmung des Schwesterchromatidaustausches (SCE) kultiviert. Unsere Ergebnisse zeigten, daß die Lymphozyten Neugeborener eine deutlich niedrigere spontane SCE-Rate aufweisen als mütterliche Zellen. Mitomycin C bewirkte einen dosisabhängigen SCE-Anstieg, während Diethylstilböstrol ohne Effekt blieb. Keine Hinweise ergaben sich dafür, daß humane mütterliche Lymphozyten im Vergleich zu den Zellen Neugeborener eine unterschiedliche Sensitivität gegenüber der chemischen SCE-Induktion in vitro besäßen.

On the induction of sister chromatid exchange by diethylstilbestrol and Mitomycin C in lymphocytes of mothers and their newborn infants

Abstract

Lymphocytes of mothers and their newborn infants were treated with varying concentrations of diethylstilbestrol (10−6M, 10−5 M and 10−4M) and mitomycin C (0.003, 0.009 and 0.03 μg/ml), and the incidence of sister chromatid exchange (SCE) was determined. The results showed that the spontaneous SCE rate in the newborn is less than that of their mothers. A dose-related increase in SCEs was oberserved in cells exposed to the alkylating chemical mitomycin C, while increases in SCEs were not noted in cultures exposed to diethylstilbestrol. No different susceptibilities of lymphocytes from mothers and newborns to the chemical induction were observed.

Schlüsselwörter

Induktion Schwesterchromatidaustausch schwangere Frau Neugeborene Mitomycin C Diethylstilböstrol 

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Copyright information

© Springer 1987

Authors and Affiliations

  • Chen Xue-min
    • 1
  • Schmidt B. 
    • 2
  • Dunkelberg H. 
    • 2
  • Borneff J. 
  1. 1.Institut für UmwelthygieneTongji Medizinische UniversitätWuhan
  2. 2.Institut für Hygiene der Johannes Gulenberg-UniversitätMainz

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