Expression of the inducible nitric oxide synthase isoform in chorionic villi in the early spontaneous abortion

  • Xia Geqing
  • Sun Yongyu


To investigate the relationship between inducible nitric oxide synthase (iNOS) and the early spontaneous abortion.,in situ hybridization and immunohistochemistry were used to detect the expression of iNOS in trophoblasts in the early pregnancy with and without spontaneous abortion (group I and groupI). By light microscopy and computer color magic image analysis system (CMIAS), light density (D) and the positive cell number per statistic square (N/S)in situ hybridization were used to analyze the positive cell index, while total positive cells (N) and the positive unit (Pu) were used in immunohistochemistry. Byin situ hybridization, D and N/S in trophoblasts were 0. 35 ± 0. 028, 0. 07 ± 0. 011 respectively in group I and 0. 18 ± 0. 016,0. 015 ± 0. 003 in groupI. In terms of immunohistochemical staining, N and Pu were 0. 058 ± 0. 007, 11. 94 ± 2. 01 in group I and 0. 013 ± 0. 009, 1. 08 ± 0. 35 in groupI in trophoblasts. Significant differences existed between two groups. It is concluded that the higher nitric oxide produced by the higher expression of iNOS in trophoblasts might play an important role in the early spontaneous abortion.

Key words

iNOS nitric oxide spontaneous abortion 


  1. 1.
    Eis AL, Brockman DE, Myatt L. Immunolocalization of the inducible nitric oxide synthase isoform in human fetal membranes. Am J Reprod Immunol, 1997, 38(4): 289PubMedGoogle Scholar
  2. 2.
    Baines MG, Duclos AJ, Antecka E, Haddad EK. Decidual infiltration and activation of macrophages leads to early embryo loss. Am J Reprod Immunol, 1997, 37(6): 471bGoogle Scholar
  3. 3.
    Weetman AP. The immunology of pregnancy. Thyroid, 1999, 9(7): 643PubMedCrossRefGoogle Scholar
  4. 4.
    Athanassakis I, Aifantis I, Ranella Aet al. Inhibition of nitric oxide production rescues LPS-induced fetal abortion in mice. Nitric Oxide, 1999, 3(3): 216PubMedCrossRefGoogle Scholar
  5. 5.
    Bogdan C. Leishmaniasis: principles of the immune response and function of nitric oxide. Berl Munch Tierarztl Wochenschr, 1998, 111(11): 409PubMedGoogle Scholar
  6. 6.
    Hortelano S, Lopez-Collazo E, Bosca L. Protective effect of cyclosporin A and FK506 from nitric oxide-dependent apoptosis in activated macrophages. Br J Pharmacol, 1999, 126(5): 1139PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2000

Authors and Affiliations

  • Xia Geqing
    • 1
  • Sun Yongyu
    • 1
  1. 1.Department of Obstetrics & Gynecology, Xiehe HospitalTongji Medical CollegeWuhan

Personalised recommendations