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In vitro expression of hepatitis C virus non-structure 5 antigen in the HepG2 cell line

  • Ye Jin
  • Zeng Linglan
  • Yang Mulan
  • Luo Duande
  • Guo Jinsong
Article
  • 14 Downloads

Summary

To establish a cell line as a model system for HCV infection and propagationin vitro, a human HepG2 cell line was incubated with a HCV RNA positive serum. The sABC immunological techniques and gold-labeled colloid electron microscopy method were employed to examine the viral proteins in those cells. The HCV non-structure 5 antigen was first detected in the HepG2 cells 72 h after incubation. The antigen was continuously observed in the cytoplasm as well on the membrane of the HepG2 cells even after 1, 2, 3 and 4 weeks after incubation. The observation of HCV non-structure 5 antigen continuously expressed in the HepG2 cells strongly indicates that the cells may have been infected by HCV virus. Therefore, the HepG2 cell line may serve as a potential host for establishment of HCV infection and propagationin vitro.

Key words

hepatitis C virus infection antigen expression 

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Copyright information

© Springer 2000

Authors and Affiliations

  • Ye Jin
    • 1
  • Zeng Linglan
    • 1
  • Yang Mulan
    • 2
  • Luo Duande
    • 1
  • Guo Jinsong
    • 1
  1. 1.Department of Infectious Disease, Xiehe HospitalTongji Medical UniversityWuhan
  2. 2.Laboratory of Ultra-Pathology of Experiment CenterTongji Medical UniversityWuhan

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