Advertisement

Journal of Tongji Medical University

, Volume 7, Issue 2, pp 65–69 | Cite as

Effects of Anti-inflammatory Agent No.6 for injection on the oxidative burst of rat peritoneal macrophages

  • Chen Xi
  • Feng Xin-wei
Article
  • 14 Downloads

Summary

Anti-inflammatory Agent No.6 for injection (AI-6) is an effective anti-infective agent composed of four traditional Chinese medicinal herbs. It was demonstrated in our experiment that the agent was able to enhance oxidative burst of rat peritoneal macrophages as shown by the increased production of H2O2 and increased reduction of nitroblue tetrazolium (NBT). It seems likely that by these means AI-6 could promote the bactericidal function of macrophages. NBT reduction of macrophages was observed by both spectrophotometric quantification and electron microscopy.

Key words

macrophage traditional Chinese medicines NBT reduction H2O2 release ultrastructure 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    1983 9: 1.Google Scholar
  2. 2.
    1983;3(5): 262.Google Scholar
  3. 3.
    Pick E, Kaisari Y. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Immunol Methods 1980;38: 161–70.PubMedCrossRefGoogle Scholar
  4. 4.
    Wiernik A, et al. The effect of Intralipid on mor.onuclear and polymorphonuclear phagocytes. Am J Clin Nutr 1983;37:256–61.PubMedGoogle Scholar
  5. 5.
    Peskin AV, et al. Visualization of reactive oxygen species formation by phagocytizing macrophages. Exp Cell Res 1984;151:247–51.PubMedCrossRefGoogle Scholar
  6. 6.
    Roos D. The metabolic response to phagocytosis. Ins Weissmann G, ed. The Cell Biology of Inflammation. Amsterdam, New York, Oxford; Elsevier, 1980: 337–85.Google Scholar
  7. 7.
    1981:71–2.Google Scholar

Copyright information

© Springer 1987

Authors and Affiliations

  • Chen Xi
    • 1
  • Feng Xin-wei
    • 1
  1. 1.Department of PathcphysiologyTongji Medical UniversityWuhan

Personalised recommendations