Summary
Human vascular endothelial growth factor (VEGF) cDNA was amplified by nested polymerase chain reaction method from the HL60 cells. Then a pCD-hVEGF165 recombinant plasmid was constructed. Rabbit osteoblasts were transfected with pCD-hVEGF165 plasmid by lipofectin mediated gene transfer. The transient expressive results were detected by immunohistochemical method. It was observed that the expression of human VEGF gene was detected 72 h after transfecting distinctly.
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Cao, Y., Shuhua, Y. & Shen, Q. Human vascular endothelial growth factor cDNA cloning and expression in osteoblasts. Current Medical Science 21, 134–137 (2001). https://doi.org/10.1007/BF02888077
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DOI: https://doi.org/10.1007/BF02888077