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Journal of Tongji Medical University

, Volume 21, Issue 2, pp 134–137 | Cite as

Human vascular endothelial growth factor cDNA cloning and expression in osteoblasts

  • Yang Cao
  • Yang Shuhua
  • Qu Shen
Article

Summary

Human vascular endothelial growth factor (VEGF) cDNA was amplified by nested polymerase chain reaction method from the HL60 cells. Then a pCD-hVEGF165 recombinant plasmid was constructed. Rabbit osteoblasts were transfected with pCD-hVEGF165 plasmid by lipofectin mediated gene transfer. The transient expressive results were detected by immunohistochemical method. It was observed that the expression of human VEGF gene was detected 72 h after transfecting distinctly.

Key words

vascular endothelial growth factor gene amplification gene expression 

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Copyright information

© 2001 2001

Authors and Affiliations

  • Yang Cao
    • 1
  • Yang Shuhua
    • 1
  • Qu Shen
    • 2
  1. 1.Department of Orthopedics, Xiehe Hospital. Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan
  2. 2.Department of Biochemistry, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan

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