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Journal of Tongji Medical University

, Volume 14, Issue 1, pp 12–15 | Cite as

Experimental study on anti-tumor effect of splenocytes induced by anti-CD3 McAb, PHA and IL-2

  • Shen Guan-xin
  • Wang Xiao-lin
  • Zhu Hui-fen
  • Zhang Yue
  • Shao Jing-fang
Article
  • 21 Downloads

Summary

The proliferation of splenocytes from healthy adults was induced by anti-CD3 McAb, PHA and IL-2. The proliferative capability and anti-tumor activity as well as phenotypes of the splenocytes cultured in different medium systems were studied. The results showed that anti-CD3 McAb and PHA not only enhanced the proliferation of splenocytes induced by IL-2, but also produced synergism if used simultaneously. The expressions of CD4 and Tac of cellular surface markers were increased after splenocytes were induced by anti-CD3 McAb and PHA. The results of anti-tumor activity of LAK cells suggested that PHA had the capability to promote anti-tumor activity of LAK cells by both direct and indirect pathways, but anti-CD3 McAb indirectly promoted anti-tumor activity of LAK cells by enhancing splenocyte proliferation.

Key words

Anti-CD3 McAb PHA IL-2 LAK cells 

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References

  1. 1.
    Grimm E A, Mazumder A, Zhang H Zet al. Lymphokine-activated killer cells phenomenon. J Exp Med, 1982; 155: 1823PubMedCrossRefGoogle Scholar
  2. 2.
    Rosenberg S A, Mule J J, Spiess P Jet al. Regression of established pulmonary metastases and subcutaneous tumor mediated by the systemic administration of high dose rIL-2. J Exp Med, 1985; 161: 1169PubMedCrossRefGoogle Scholar
  3. 3.
    Schwarz R E, Hiserodt J C. The importance of splenectomy for the adoptive immunotherapy of cancer. Med Hypotheses, 1989; 28: 165PubMedCrossRefGoogle Scholar
  4. 4.
    Garbrecht F C, Russo C and Weksler M E. Long-term growth of human T cell lines and cloness on anti-CD3 antibody-treated tissue culture plates. J Immunol Methods, 1988; 107: 137PubMedCrossRefGoogle Scholar
  5. 5.
    Londei M, Grubeck-Loebenstein B, De Berardings Pet al. Efficient propagation and cloning of human T cells in the absence of antigen by means of OKT3, interleukn-2 and antigen-presenting cells. Scand J Immunol, 1988; 27: 35PubMedCrossRefGoogle Scholar
  6. 6.
    1988; 4(4): 217Google Scholar
  7. 7.
    1992; 12 (3): 139Google Scholar
  8. 8.
    1991; 11(1): 35Google Scholar
  9. 9.
    Schwinzep R, Franklin R A and Domenico Jet al. Monoclonal antibodies directed to different epitopes in the CD3-TCR complex induce different states of competence in resting human T cells. J Immunol 1992; 148: 1322Google Scholar
  10. 10.
    1990; 6(1): 22Google Scholar
  11. 11.
    Eichmann K, Jonsson J I, Falk Iet al. Effective activation of resting mouse T lymphocytes by cross-linking submitogenic concentration of the cell antigen receptor with enther Lyt-2 or L3T4. Eur J Immunol, 1987; 17: 643PubMedCrossRefGoogle Scholar

Copyright information

© Springer 1994

Authors and Affiliations

  • Shen Guan-xin
    • 1
  • Wang Xiao-lin
    • 1
  • Zhu Hui-fen
    • 1
  • Zhang Yue
    • 1
  • Shao Jing-fang
    • 1
  1. 1.Department of Immunology, Experimental Research Center of MedicineTongji Medical UniversityWuhan

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