Journal of Tongji Medical University

, Volume 16, Issue 2, pp 70–74 | Cite as

Construction of expressing plasmids of recombinant FN polypeptides with bifunctional-domain and the characterization of the products expressed inE. coli

  • Feng Zuohua
  • Zhang Guimei
  • Li Dong
  • Zhang Hui


Two expressing plasmids have been constructed and used to express two bifurictional-domain recombinant polypeptides of human fibronectin (FN) inE. coli. One was CH50 (Pro1239-Ser1515 of FN linked with Ala1690-Thr1960 of FN through Met) and the other was CH56 (Pro1239-Thr1960 of FN). Both of two polypeptides were capable of binding heparin and were purified by heparin-agarose affinity chromatography. The purified products were capable of binding cells. The production of CH50 and CH56 polypeptides provided a fundamental basis for further study of the anti-metastatic function of recombinant fibronectin polypeptides.

Key words

fibronectin recombinant polypeptide metastasis 


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Copyright information

© Springer 1996

Authors and Affiliations

  • Feng Zuohua
    • 1
  • Zhang Guimei
    • 1
  • Li Dong
    • 1
  • Zhang Hui
    • 1
  1. 1.Department of Medical Molecular BiologyTongji Medical UniversityWuhan

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