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Journal of Tongji Medical University

, Volume 16, Issue 2, pp 70–74 | Cite as

Construction of expressing plasmids of recombinant FN polypeptides with bifunctional-domain and the characterization of the products expressed inE. coli

  • Feng Zuohua
  • Zhang Guimei
  • Li Dong
  • Zhang Hui
Article

Summary

Two expressing plasmids have been constructed and used to express two bifurictional-domain recombinant polypeptides of human fibronectin (FN) inE. coli. One was CH50 (Pro1239-Ser1515 of FN linked with Ala1690-Thr1960 of FN through Met) and the other was CH56 (Pro1239-Thr1960 of FN). Both of two polypeptides were capable of binding heparin and were purified by heparin-agarose affinity chromatography. The purified products were capable of binding cells. The production of CH50 and CH56 polypeptides provided a fundamental basis for further study of the anti-metastatic function of recombinant fibronectin polypeptides.

Key words

fibronectin recombinant polypeptide metastasis 

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References

  1. 1.
    Kimizuka F, Taguchi Y, Ohaate Yet al. Production and characterization of functional domains of human fibronection expressed in Escherichia coli. J Biochem, 1991, 110: 284PubMedGoogle Scholar
  2. 2.
    Saiki I, Makabe T, Yoneda Jet al. Inhibitory effect of fibronection and its recombinant polypeptides on the adhesion of metastatic melanoma cells to laminin. Jpn J Cancer Res, 1991, 82: 1–112Google Scholar
  3. 3.
    Matsumoto Y, Saiki Y, Makabe Tet al. Inhibitory effect of antimetastatic fusion polypeptide of human fibronectin on tumor cell adhesion to extracellular matrices. Jpn J Cancer Res, 1991, 82: 1–130Google Scholar
  4. 4.
    Yoneda J, SaikiI, Kobayashi Het al. Inhibitory effect of recombinant fibronectin polypeptides on the adhesion ofliver-metastatic lymphoma cells to hepatic sinusoidal endothelial cells and tumor invasion. Jpn J Cancer Res, 1994, 85: 723PubMedGoogle Scholar
  5. 5.
    Saiki I, Murata J, Makabe Tet al. Inhibition of lung metastasis by synthetic and recombinant fragments of human fibronectin witn functional domains. Jpn J Cancer Res, 1990, 81: 1–003Google Scholar
  6. 6.
    Saiki I, Matsumoto Y, Murata Jet al. Recombinant fusion polypeptide with Cell- and heparin-binding domains of fibronectin inhibits liver metastasis of L5178Y-ML25 lymphoma cells. Jpn J Cancer Res, 1991, 82: 1–120Google Scholar
  7. 7.
    Kornblihtt A, Umezawa K, Vibe-Pedersen Ket al. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. The EMBO J, 1985, 4(7): 1–755Google Scholar
  8. 8.
    Sambrook J, Fritch EF, Maniatis T. Molecular Cloning, a laboratory manu. 2nd ed, New York: Cold Spring Harbor Laboratory Press, 1989. 1.21–1.87Google Scholar
  9. 9.
    Hynes R O. Fibronectins. New York: Springer-Verlag New York Inc, 1990. 113–115Google Scholar
  10. 10.
    Beezhold DH, Personius C. Fibronectin fragments stimulate tumor necrosis factor secretion by human monocytes. J Leukoc Biol, 1992, 51 (1):59PubMedGoogle Scholar

Copyright information

© Springer 1996

Authors and Affiliations

  • Feng Zuohua
    • 1
  • Zhang Guimei
    • 1
  • Li Dong
    • 1
  • Zhang Hui
    • 1
  1. 1.Department of Medical Molecular BiologyTongji Medical UniversityWuhan

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