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Journal of Tongji Medical University

, Volume 12, Issue 3, pp 179–182 | Cite as

Detection of surface antigen expression of pre- and postcultured normal adult’s bone marrow cells by using ABC technique and its significance

  • Chen Yan
  • Yu Dong-jiao
  • Li Chong-yu
  • Zou Ping
  • Wang Chun
  • Li Yao
Article

Summary

The expression of surface antigens on both normal adult’s bone marrow and multipotential progenitor cells (CFU-Mix) was detected by use of ABC technique. The culture system of CFU-Mix was an ideal model for studying the differentiation direction of hematopoietic cells. The monoclonal antibodies (McAb) CD3, CD4, CD8specific for T-lymphocytic lineage, CD22 for B-lymphocytic lineage and CD11, CD13, CD14 for granulocytomonocytic lineage were all demonstrated in certain proportion in cultured CFU-Mix, which might be associated with the control of hematopoiesis. A decrease in the positive rate of OKT, and CD34 in cultured cells might be related to the differentiation of hematopoietic cells. SZ-2 and SZ-21 could contribute to the identification of the presence of megakaryocytes in CFU-Mix.

Key words

ABC technique bone marrow cell CFU-Mix monoclonal antibody 

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Copyright information

© Springer 1992

Authors and Affiliations

  • Chen Yan
    • 1
  • Yu Dong-jiao
    • 1
  • Li Chong-yu
    • 1
  • Zou Ping
    • 1
  • Wang Chun
    • 1
  • Li Yao
    • 1
  1. 1.Institute of Hematology, Xiehe HospitalTongji Medical UniversityWuhan

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