Journal of Tongji Medical University

, Volume 19, Issue 3, pp 212–214 | Cite as

Effect of IL-Iβ and TNF-α on the expression of monocyte chemotactic protein-1 in endometriotic cells

  • Gao Ying
  • Luo Lilan
  • He Fuxian


To investigate the clinical significance of monocyte chemotactic protein-1 (MCP-1) produced by endometriotic tissues, the endometriotic tissues were taken from 15 patients with endometriosis. MCP-1 mRNA and MCP-1 protein were determined by dot blot analysis and enzyme linked immunosorbent assay (ELISA) in endometriotic cells cultured with or without interleukin-1β (IL-lβ, 2 μg/L), tumor necrosis factor-α (TNF-α, 20 g/L). After exposure to IL-1β or TNF-α, the expression of MCP-1 mRNA in the endometriotic cells (8.635 ±0.826, 7.031 ±0.970, respectively) were significantly higher than that in the control group (4.482±0.435, P<0.05); The expression of MCP-1 protein in IL-lβ and TNF-α group was 4.52±0.09 μg/L,2.87±0.27 μg/L, respectively, which were significantly higher than 1.74±0.16 μg/L in control (P<0.01). The results suggested that IL-l\ and TNF-α could up-regulate the expression of MCP-1 in endometriotic cells, which might be related to the development of endometriosis.

Key words

endometriosis cell culture MCP-1 IL-l\ TNF-α 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Weil S J, Wang S, Perez M C. Chemotaxis of macrophages by a peritoneal fluid of patients with endometriosis. Fertil Steril, 1997, 67:865PubMedCrossRefGoogle Scholar
  2. 2.
    Dodds W, Miller F, Feidman Cet al. The effect of preovulatory peritoneal fluid from cases of endometriosis on murinein vitro fertilization, enbryo development, oviduct transport and implantation. Am J Obstet Gynecol, 1992, 166:219PubMedGoogle Scholar
  3. 3.
    Taketani Y, Kuo T M, Mizuno M. Comparison of cytokine levels and embryo toxicity in peritoneal fluid in infertil women with untreated and treated endometriosis. Am J Obstet Gynecol, 1992, 67:765Google Scholar
  4. 4.
    Randolph G J, Furie M B. A soluble gradient of endogenous monocyte chemoattractant protein-1 promotes the transendothelial migration of monocytes in vitro. J Immunol, 1995, 155:3610PubMedGoogle Scholar
  5. 5.
    Yoshimera T, Leonard E J. Identification of high affinity receptors for human monocyte chemoattractant protein-1 on human monocyte. J Immunol, 1990, 145:292Google Scholar
  6. 6.
    Jolicoeur C, Boutouil I. Increased expression of monocyte chemotactic protein-1 in the endometrium of woman with endometriosis. Am J Pathol, 1998, 152:125PubMedGoogle Scholar

Copyright information

© Springer 1999

Authors and Affiliations

  • Gao Ying
    • 1
  • Luo Lilan
    • 1
  • He Fuxian
    • 1
  1. 1.Department of Obstetrics and Gynecology, Xiehe HospitalTongji Medical UniversityWuhan

Personalised recommendations