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Journal of Tongji Medical University

, Volume 19, Issue 3, pp 170–174 | Cite as

Construction and production of fluorescent papillomavirus-like particles

  • Peng Shiwen
  • Zhou Jian
  • Ian H. Frazer
Article

Summary

The green fluorescent protein (GFP) from the jellyfishAequorea victoria has attracted widespread interest since it was demonstrated to be fluorescent in vivo when expressed in other organisms. In order to investigate papillomavirus life cycle which hampered by the unavailability of conventional cell culture system, we constructed a chimeric bovine papillomavirus (BPV) type 1 virus-like particles (VLPs) containing GFP. It was found that fluorescent VLPs could be assembled from L2 protein in which GFP is inserted into the N-terminal region of L2 (aa 88). The fluorescent VLPs could also be assembled from a GFP/L2 fusion protein in which part of the L2 sequence had been deleted.In vitro, fluorescent VLPs could bind to CV-1 cells, and this VLP/cell interaction could be analyzed by FACS assay. These results demonstrated that GFP could incorporate into BPVl VLPs without disruption of the VLP structure. Fluorescent VLPs might be a useful tool for study of papillomavirus virus/cell interaction.

Key words

papillomavirus virus-like particles green fluorescent protein 

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Copyright information

© Springer 1999

Authors and Affiliations

  • Peng Shiwen
  • Zhou Jian
    • 1
    • 2
  • Ian H. Frazer
  1. 1.Department of Pediatrics, Xiehe HospitalTongji Medical UniversityWuhan
  2. 2.Center for Immunology and Cancer ResearchUniversity of Queensland, Princess Alexandra HospitalWoolloongabbaAustralia

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