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Folia Microbiologica

, Volume 11, Issue 5, pp 364–372 | Cite as

Dehydrogenase activity and toxinogenesis of a production strain ofCorynebacterium diphtheriae during submerged cultivation

  • Kateřina Nekvasilová
  • J. Fousek
Article
  • 23 Downloads

Abstract

A simple biochemical procedure was obtained for studying metabolism ofCorynebacterium diphtheriae during submerged cultivation based on the modification of the assay of dehydrogenase activity using 2,3,5-triphenyltetrazolium chloride as redox indicator. Results obtained by the estimation of the dehydrogenase activity using TTC are in a good accordance with oxygen consumption assayed manometrically. By following dehydrogenase activity in submerged cultivations of a production strain ofCorynebacterium diphtheriae PW8-Weissensee we found that a massive toxin production is connected with the decrease of the activity of cells. This fall of activity occurs yet during the exponential phase of growth. Especially a sudden fall of succindehydrogenase activity exactly indicates the beginning of a considerable toxin accumulation in the medium. The presence of inhibitory concentrations of iron ions in the medium not only increases the level of dehydrogenase activity but changes its whole kinetics. A retarded and irregular fall of the activity occurs instead of a sharp one typical for good toxin production.

Keywords

Dehydrogenase Activity Diphtheria Toxin Production Diphtheria Toxin Viable Cell Count 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1966

Authors and Affiliations

  • Kateřina Nekvasilová
    • 1
  • J. Fousek
    • 1
  1. 1.Institute of Sera and VaccinesPrague 10

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