Journal of Tongji Medical University

, Volume 20, Issue 1, pp 32–35 | Cite as

Effects of cigarette smoke extract on E-cadherin expression in cultured airway epithelial cells

  • Wang Xi
  • Wu Renling
  • Hao Tianling
  • Chen Fang


To investigate whether the change of E-cadherin (ECD) expression plays a role in the injury and repair of airway epithelial cells (AEC) caused by smoking, porcine AECs were cultured by using an enzyme-dispersed method. After exposure of the AECs to cigarette smoke extract (CSE), the ECD expression in the cells was detected by using immunocytochemistry and in situ hybridization. The results showed that ECD was distributed on the plasma membrane at the cell junctions of AECs. After exposure to 20 % CSE, the membranous ECD expression was decreased, the cytoplasmic ECD expression was increased (P < 0.01) as the exposure time went on. But the content of ECD mRNA in the AECs did not chang. It suggests that the change of ECD expression is regulated at the posttranslational level and plays a role in the injury and repair of AEC caused by smoking.

Key words

airway epithelial cell E-cadherin smoke inhalation injury 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    1998, 20:42Google Scholar
  2. 2.
    Higgins M. Epidemiology of COPD. State of the art. Chest, 1984, 85: 3sPubMedCrossRefGoogle Scholar
  3. 3.
    Wu R, Yankaskas J, Cheng Eet al. Growth and differentiation of human nasal epithelial cells in culture. Am Rev Respir Dis, 1985, 132: 311PubMedGoogle Scholar
  4. 4.
    Wu R, Nolan E, Turner C. Expression of tracheal differentiated function in serum-free hormone-supplemented medium. J Cell Physiol, 1985, 125: 167PubMedCrossRefGoogle Scholar
  5. 5.
    Nakamura Y, Romberger D J, Tate Let al. Cigarette smoke inhibits lung fibroblast proliferation and chemotaxis. Am J Respir Crit Care Med, 1995, 151: 1497PubMedGoogle Scholar
  6. 6.
    Sambrook J, Fritsch E F, Maniatis Tet al. 1993.16–60, 309–325Google Scholar
  7. 7.
    1996, 48:190Google Scholar
  8. 8.
    Roche W R, Montefort S, Baker Jet al. Cell adhesion molecules and the bronchial epithelium. Am Rev Respir Dis, 1993, 148: s79PubMedGoogle Scholar
  9. 9.
    1998, 7:302Google Scholar
  10. 10.
    Hanby A M, Chinery R, Porlsom Ret al. Downregulation of E-cadherin in the reparative epithelium of the human gastrointestinal tract. Am J Pathol, 1996, 148: 723PubMedGoogle Scholar
  11. 11.
    Hulsken J, Birchmeier W, Behrens J. E-cadherin and APC compete for the interaction with β-catenin and the cytoskelelton. J Cell Biol, 1996, 127: 2061CrossRefGoogle Scholar

Copyright information

© Springer 2000

Authors and Affiliations

  • Wang Xi
    • 1
  • Wu Renling
    • 1
  • Hao Tianling
    • 2
  • Chen Fang
    • 1
  1. 1.Department of Pathology, School of Basic Medical SciencesTongji Medical UniversityWuhan
  2. 2.Pulmonary Disease Laboratory, Ministry of HealthTongji Medical UniversityWuhan

Personalised recommendations