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Folia Microbiologica

, Volume 16, Issue 1, pp 35–40 | Cite as

Role of endogenous tryptophan during submerged fermentation of ergot alkaloids

  • Z. Řeháček
  • J. Kozová
  • A. Řičicová
  • J. Kašlík
  • P. Sajdl
  • S. Švarc
  • S. C. Basappa
Article

Abstract

Shake flask cultures ofClaviceps paspali (Stev. et Hall) andClaviceps purpurea (Fr.) Tul. on simple synthetic medium have been studied. Both strains grown in the absence of added tryptophan accumulate extra endogenous tryptophan. A certain concentration of cell-pool tryptophan is needed to promote alkaloid synthesis. Alkaloid production commences while tryptophan synthetase activity is increasing. In the alkloid-producing phase cell-pool tryptophan shows a single minimum while the change in level of cell-protein tryptophan is negligible. Alkaloid formation is suggested to reflect a regulatory device to keep endogenous tryptophan balanced. By adding amitrole the alkaloid spectrum is changed. The tryptophan-histidine cross-pathway probably serves a useful function inthe biosynthesis of ergot alkaloids.

Keywords

Tryptophan Alkaloid Ergot Alkaloid Alkaloid Production Amitrole 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bu'Lock, J. D., Barr, J. G.:A regulation mechanism linking tryptophan uptake and synthesis with ergot alkaloid synthesis in Claviceps. Lloydia 31: 342, 1968.Google Scholar
  2. Casselton, P. J.:Further observation on the inhibition of Prototheca zopfii grouth by 3-amino-1,2,4-triazole. Physiol. Plantarum 19: 411, 1966.CrossRefGoogle Scholar
  3. Feigelson, P., Greengard, O.:Regulation of liver tryptophan pyrrolase activity. J. biol. Chem. 237: 1908, 1962.PubMedGoogle Scholar
  4. Floss, H. G., Mothes, U.:Über den Einfluss von Tryptophan und analogen Verbindungen auf die Biosynthese von Clavinalkaloiden in saprophytischer Kultur. Arch. Mikrobiol. 48: 213, 1964.PubMedCrossRefGoogle Scholar
  5. Hilton, J. L.:Modes of action of 3-amino-1,2,4-triazole. Current status. (In: Isotopes in weed research. Int. Atomic Energy Ageney, Vienna,Google Scholar
  6. Jensen, R. A.:Metabolic interlock. Regulatory interactions exerled between biochemical pathways. J. biol. Chem. 244: 2816, 1969.PubMedGoogle Scholar
  7. Knox, W. E.:Tryptophan oxidation. (In: Colowick, S. P. Kaplan, O.: Methods in Enzymology. Vol. II. Acad. Press, New York p. 246, 1955.Google Scholar
  8. Knox, W. E., Mehler, A. H.:The adaptive increase of the tryptophan peroxidase-oxidase system of liver. Science 113: 237, 1951.PubMedCrossRefGoogle Scholar
  9. Kobel, H., Schreier, E., Rutschmann, J.:Ergot alkaloids. 60. 6-methyl delta 8,9-ergoline-8-car-boxylic acid, a new ergoline derivative from cultures of Claviceps paspali. Helv. Chim. Acta 47: 1052, 1961.CrossRefGoogle Scholar
  10. Lingens, F., Goebel, W., Uesseler, H.:Nachweis von Enzymen zur Biosynthese der cromatischen Aminosaeuren in Claviceps paspali. Naturwissenschaften 54: 141, 1967.PubMedCrossRefGoogle Scholar
  11. Lowry, O. H., Rosebrough, N. H., Farr, A. L., Randall, R. J.Protein measurement with the Folin phenol reagent. J. biol. Chem. 193: 265, 1951.PubMedGoogle Scholar
  12. Mothes, K.:Biogenesis of alkaloids and the problem of chmmotaxonomy. Lloydia 29: 156, 1966.Google Scholar
  13. Roeder, K., Mutschler, E., Rochelmeyer, H.:Über die quantitative Bestimmung der Mutterkornalkaloide nach duennschichtchromatographischer Trennung. Pharm. Acta Helv. 42: 407, 1967.Google Scholar
  14. Teuscher, E.:Über Bedingungendder Biosynthese von Clavinalkaloiden in saprophytischer Kultur. Pharmazie 16: 570, 1961.PubMedGoogle Scholar
  15. Trembley, G. C., Gottlieb, J. A., Knox, W. E.:Induction by L-tryptophan and an analogue, α-methyl-DL-tryptophan, of the enzymes catabolizing L-tryptophan in Pseudomonas. J. Bacteriol. 93: 168, 1967.Google Scholar
  16. Weygand, F., Floss, H. G.:The biogenesis of ergoi alkaloids. Angew. Chem. Intern. Ed. 2: 243, 1963.CrossRefGoogle Scholar
  17. Yanofsky, C.:Tryptophan synthetase from Neurospora. (In: Colowick, S. P. Kaplan, O.: Methods in Enzymology. Vol. II. Acad. Press, New York p. 233, 1955.CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1971

Authors and Affiliations

  • Z. Řeháček
    • 1
  • J. Kozová
    • 1
  • A. Řičicová
    • 1
  • J. Kašlík
    • 1
  • P. Sajdl
    • 1
  • S. Švarc
    • 1
  • S. C. Basappa
    • 1
  1. 1.Department of Biogenesis of Natural Substances, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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