Chinese Science Bulletin

, 45:1122 | Cite as

Localization of orphan receptor TR3 mRNA in early developmental follicles in rat

  • Haizhen Liu
  • Yixun Liu


Byin situ hybridization, the localization of orphan receptor TR3 mRNA has been observed in early developmental follicles. TR3 mRNA is first expressed in the ovarian interstitial cells on day 2 after birth, and then in granulosa cells (GC) in primary follicles on day 4. The expression level of TR3 mRNA in GC increases following the follicular development. Its higher expression can be observed in the outer layer of GC and inner layer of theca cells (TC) on day 6, where the cells present active proliferation and differentiation. The expression of TR3 is in an increasing manner until the large antral follicles on day 30. The mRNA is only expressed in the healthy, but not atretic follicles in adult rat ovaries. Injection of epidermal growth factor (EGF) has dramatically enhanced its expression in the early stage of developmental follicles. It is therefore suggested that TR3 may play a role in regulating growth and differentiation of ovarian somatic cells in the early stage, and its expression is regulated by EGF.


orphan receptor TR3 in situ hybridization follicular development granulosa cells epidermal growth factor 


  1. 1.
    Tsai, M. J., O’Malley, B. W., Molecular mechanism of action of steriod/thyroid receptor superfamily members, Ann. Rev. Biochem., 1994,63: 451.PubMedCrossRefGoogle Scholar
  2. 2.
    Hazel, G. F., Nathans, D., Lau, L. F., A gene inducible by serum growth factors encodes a member of the steroid and thyroid hormone receptor superfamily, Proc. Natl. Acad. Sci. USA, 1988, 85: 8444.PubMedCrossRefGoogle Scholar
  3. 3.
    Mu, X. M., Liu, Y. X., Localization and expression of TR3 orphan receptor protein and its mRNA in rat testis, Chinese Science Bulletin, 1998,43: 146.CrossRefGoogle Scholar
  4. 4.
    Mu, X. M., Liu, Y. X., Localization and expression of TR3 orphan receptor in mouse testis, Acta Physiol. Sinica, 1998, 50: 439.PubMedGoogle Scholar
  5. 5.
    Mu, X. M., Liu, Y. X., Expression of TR3 orphan mRNA in rhesus monkey testis, Chinese Science Bulletin, 1999, 44: 927.CrossRefGoogle Scholar
  6. 6.
    Willams, G. T., Lau, L. F., Activation of the inducible orphan receptor gene Nur77 by serum growth factor: dissociation of immediate-early responses, Mol. Cell Biol., 1993, 13: 6124.Google Scholar
  7. 7.
    Davis, I. J., Lau, L. F., Endocrine and neurogenic regulation of the orphan receptors Nur 777 and Nurr-1 in the adrenal glands, Mol. Cell Biol., 1994, 14: 3469.PubMedGoogle Scholar
  8. 8.
    Lee, S. L., Wesselschmidt, R. L., Linette, G. P. et al., Unimpaired thymic and peripheral T cell death in mice lacking the nuclear receptor NGF-IB (Nur 77), Science, 1995, 269: 532.PubMedCrossRefGoogle Scholar
  9. 9.
    Woronicz, J. D., Calnan, B., Ngo, V. et al., Requirement for the orphan steroid receptor Nur 77 in apoptosis of T cell hybridomas, Nature 1994, 367: 277.PubMedCrossRefGoogle Scholar
  10. 10.
    Grawford, P. A., Sadovsky, Y., Woodson, K. et al., Adrenocortical function and regulation of the steroid 21-hydro-xylase gene in NGF-IB-deficient mice, Mol. Cell Biol., 1995, 15: 4331.Google Scholar
  11. 11.
    Wilson, T., Mouw, A. R., Weaver, C. A. et al., The orphan receptor NGF-IB regulates expression of the gene encoding steroid 21-hydroxylase, Mol. Cell Biol., 1993, 13: 861.PubMedGoogle Scholar
  12. 12.
    Lin, H. H., Tu, Z. J., Ann, D. K., Involvement of nuclear orphan receptor NGF-IB in transcriptional activation of salivary-specific R 15 gene by cAMP, J. Biol. Chem., 1996, 271: 27637.PubMedCrossRefGoogle Scholar
  13. 13.
    Paulsen, R. E., Weaver, C. A., Fahmer, T. J. et al., Domain regulating transcriptional activity of the inducible orphan receptor NGF-IB, J. Biol. Chem., 1992, 267: 16491.PubMedGoogle Scholar
  14. 14.
    Oktay, K., Schenken, R. S., Nelson, J. F., Proliferating cell nuclear antigen marks the initiation of follicular growth in the rat, Biol. Reprod., 1995, 53: 295.PubMedCrossRefGoogle Scholar
  15. 15.
    Lee, H. J., Kokonitis, J., Wang, K. C. et al., The use of a DNA-binding domain replacement method for the detection of a potential TR3 orphan receptor response element in the mouse mammary tumor virus long terminal repeat, Biochem. Biophys. Res. Commun., 1993, 194: 97.PubMedCrossRefGoogle Scholar
  16. 16.
    Mu, X. M., Liu, Y. X., Mechanism of the interaction between orphan receptor TR3 andcis-acting element inCNTFR2 gene, Chin. J. Biochem. Mol. Biol., 1998, 14: 485.Google Scholar
  17. 17.
    Ryseck, R. P., Macdonald-Bravo, H., Mattei, M. G. et al., Structure, mapping and expression of a growth factor inducible gene encoding a putative nuclear hormonal binding receptor, EMBO J., 1989, 8: 3327.PubMedGoogle Scholar
  18. 18.
    Eppig, J. J., O’Brien, M. J., Developmentin vitro of mouse oocytes from primordial follicles, Biol. Reprod., 1996, 54: 197.PubMedCrossRefGoogle Scholar
  19. 19.
    Guo, C. X., Liu, Y. X., Expression and regulation of orphan receptor TR2 mRNA in germ cell apoptosis in the cryptorchild testis of rat and rhesus monkey, Chinese Science Bulletin, 2000, 45(8): 720.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2000

Authors and Affiliations

  1. 1.State Key Laboratory of Reproductive Biology, Institute of ZoologyChinese Academy of SciencesBeijingChina

Personalised recommendations