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Chinese Science Bulletin

, Volume 45, Issue 6, pp 528–532 | Cite as

Molecular verification of the integration ofTripsacum dactyloides DNA into wheat genome through wide hybridization

  • Jiwen Qiu
  • Demin Jin
  • Chuanyou Li
  • Jianhang Jia
  • Ping Ouyang
  • William Tai
  • Bin Wang
  • Dawei Li
Notes
  • 25 Downloads

Abstract

RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L.2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplifiedTripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact thatTripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferringTripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.

Keywords

bread wheat Tripsacum dactyloides wide hybridization RAPD RFLP 

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References

  1. 1.
    Zenkteler, M., Nitzsche, W., Wide hybridization experiments in cereals, Thero. Appl. Genet., 1984, 68: 311.CrossRefGoogle Scholar
  2. 2.
    Laurie, D. A., Bennett, M. D., Wheat × maize hybridization, Can. J. Genet. Cytol., 1986, 28: 313.Google Scholar
  3. 3.
    Laurie, D. A., Bennett, M. D., Cytological evidence for fertilization in haploid wheat × Sorghum crosses, Plant Breeding, 1988, 100: 73.CrossRefGoogle Scholar
  4. 4.
    Ahmad, F., Comeau, A., Wheat × pearl millet hybridization: consequence and potential, Euphytica, 1990, 50: 181.CrossRefGoogle Scholar
  5. 5.
    Ushiyama, T., Shimizu, T., Kuwabara, T., High frequency of haploid production of wheat through intergeneric cross with teosinte, Japan J. Breeding, 1991, 41: 353.Google Scholar
  6. 6.
    Riera-Lizarazu, O., Mujeeb-Kazi, A., Polyhaploid production in the Triticeae: wheat ×Tripsacum crosses. Crop Sci., 1993, 33: 973.Google Scholar
  7. 7.
    Li, D. W., Qiu, J. W., Ouyang, P. et al., High frequencies of fertilization and embryo formation in hexaploid wheat ×Tripsacum dactyloides crosses, Theo. Appl. Genet, 1996, 92: 1103.CrossRefGoogle Scholar
  8. 8.
    Kindiger, B., Dewald, C. L., The reproductive versatility of eastern gamagrass, Crop Sci., 1997, 37: 1351.Google Scholar
  9. 9.
    Li, D.W., Qiu, J. W., Ouyang, P. et al., A new high-potent pollen donor in wheat intergeneric crosses-Tripsacum dactyloides, Acta Botanica Sinica (in Chinese), 1994, 36(suppl.): 155.Google Scholar
  10. 10.
    Ouyang, P., Li, D. W., Qiu, J. W., Wheat haploid and hybrid plants obtained from intergeneric crosses of wheat withTripsacum dactyloides, Chinese Science Bulletin, 1996, 41(17): 1616.Google Scholar
  11. 11.
    Xie, W. W., Wang, B., Lei, B. J. et al., Introduction of exogenous wild soybean DNA into cultivated soybean and RAPD molecular verification, Science in China, Ser. B, 1995, 38(1):1195.Google Scholar
  12. 12.
    Lei, B. J., Lu, C. H., Qian, H. et al., Early maturing lines obtained through foreign total DNA introduction in soybean, Acta Agronomica Sinica (in Chinese), 1996, 22(2): 173.Google Scholar
  13. 13.
    Li, D. W., Ouyang, P., Yao, Q. X. et al., Wide hybridization betweenTriticum aestivum andTripsacum dactyloides II. Immature embryo culture, Acta Genetica Sinica, 21(6): 474.Google Scholar
  14. 14.
    McCouch, S. R., Kochert, G., Yu, Z. H. et al., Molecular mapping of rice chromosomes, Theor, Appl. Genet., 1998, 76: 815.CrossRefGoogle Scholar
  15. 15.
    Li, S. T., Zhang, Z. T., Wang, B. et al., Tagging the Pm4a gene in NILs by RAPD analysis, Acta Genetica Sinica, 1995, 22(2): 103.Google Scholar
  16. 16.
    Xie, W. W., Kang, C. H., Wang, J. Z., Molecular characterization of a new type of cytoplasmic male sterile sugar beet. Science in China, Ser. C, 1996, 39(1): 54.Google Scholar

Copyright information

© Science in China Press 2000

Authors and Affiliations

  • Jiwen Qiu
    • 1
  • Demin Jin
    • 2
  • Chuanyou Li
    • 2
  • Jianhang Jia
    • 2
  • Ping Ouyang
    • 1
  • William Tai
    • 3
  • Bin Wang
    • 2
  • Dawei Li
    • 1
  1. 1.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of GeneticsChinese Academy of SciencesBeijingChina
  2. 2.Plant Biotechnology Laboratory, Institute of GeneticsChinese Academy of SciencesBeijingChina
  3. 3.Institute for Global Chinese AffairsUniversity of MarylandUSA

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