Study on angiogenesis factor of human osteosarcoma
Angiogenesis factor of human osteosarcoma was partially purified and its biological features were studied. The active peptide with 8000 to 10 000 u molecular weight in the conditioned medium obtained from the cultivation of human osteosarcoma cells were partially purified by ultrafiltration, chromatography and dialysis. The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig aorta thoracic endothelial cells. The results showed that the chromatography fractions of 4 to 6 could significantly promote the proliferation of the endothelial cells. It was suggested that the human osteosarcoma cells could synthesize and secrete angiogenesis factor with a molecular weight of 8000 to 10 000 u.
Key wordsosteosarcoma angiogenesis factor
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- 3.Arira Kawai. A newly established human osteosarcoma cell line with osteoblastic properties. Clin Orthop Related Res, 1990, 259:256Google Scholar
- 5.1990, 19(1):4Google Scholar
- 8.Yamune Takashi. Establishment and characterization of cell lines derived from a human osteosarcoma. Clin Orthop Related Res, 1985, 199:261Google Scholar