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Study on angiogenesis factor of human osteosarcoma

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Summary

Angiogenesis factor of human osteosarcoma was partially purified and its biological features were studied. The active peptide with 8000 to 10 000 u molecular weight in the conditioned medium obtained from the cultivation of human osteosarcoma cells were partially purified by ultrafiltration, chromatography and dialysis. The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig aorta thoracic endothelial cells. The results showed that the chromatography fractions of 4 to 6 could significantly promote the proliferation of the endothelial cells. It was suggested that the human osteosarcoma cells could synthesize and secrete angiogenesis factor with a molecular weight of 8000 to 10 000 u.

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References

  1. Folkman J, Klagsbrun M. Angiogenic factor. Science, 1987, 235:442

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Fournier B, Price PA. Characterization of a new human osteosarcoma cell line OHS-4. J Cell Biol, 1991, 114(3): 577

    Article  CAS  PubMed  Google Scholar 

  3. Arira Kawai. A newly established human osteosarcoma cell line with osteoblastic properties. Clin Orthop Related Res, 1990, 259:256

    Article  Google Scholar 

  4. Shapiro R, Strydom D J. Isolation of angiogenin from normal human plasma. Biochemistry, 1987, 26:5141

    Article  CAS  PubMed  Google Scholar 

  5. 1990, 19(1):4

  6. Okabe T, Takaku F. A macrophage factor that stimulates the proliferation of vascular endothelial cells. Biochem Biophys Res Commun, 1986, 134:344

    Article  CAS  PubMed  Google Scholar 

  7. Form D M, Pratt B M. Endothelial cell proliferation during angiogenesis. Lab Invest, 1986, 55(5):521

    CAS  PubMed  Google Scholar 

  8. Yamune Takashi. Establishment and characterization of cell lines derived from a human osteosarcoma. Clin Orthop Related Res, 1985, 199:261

    Google Scholar 

  9. Schott R J, Morrow L A. Growth factors and angiogenesis. Cardiovascular Res, 1993, 27:1155

    Article  CAS  Google Scholar 

  10. Fell J W, Strydom D J. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells. Biochemistry, 1985, 24(20):5480

    Article  Google Scholar 

  11. Folkman J, Haudenschild C. Angiogenesis in vitro. Nature, 1980, 288:551

    Article  ADS  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Orthopedics, Tongji Hospital, Tongji Medical University, 430030, Wuhan

    Wu Hua (Associate Professor) & Wang Taiyi

  2. Department of Pathology, School of Basic Medical Sciences, Tongji Medical University, 430030, Wuhan

    Deng Zhongdua & Chen Duo’en

Authors
  1. Wu Hua
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  2. Wang Taiyi
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  3. Deng Zhongdua
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  4. Chen Duo’en
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Hua, W., Taiyi, W., Zhongdua, D. et al. Study on angiogenesis factor of human osteosarcoma. Current Medical Science 20, 227–230 (2000). https://doi.org/10.1007/BF02886999

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  • DOI: https://doi.org/10.1007/BF02886999

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