Journal of Tongji Medical University

, Volume 20, Issue 3, pp 227–230 | Cite as

Study on angiogenesis factor of human osteosarcoma

  • Wu Hua
  • Wang Taiyi
  • Deng Zhongdua
  • Chen Duo’en


Angiogenesis factor of human osteosarcoma was partially purified and its biological features were studied. The active peptide with 8000 to 10 000 u molecular weight in the conditioned medium obtained from the cultivation of human osteosarcoma cells were partially purified by ultrafiltration, chromatography and dialysis. The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig aorta thoracic endothelial cells. The results showed that the chromatography fractions of 4 to 6 could significantly promote the proliferation of the endothelial cells. It was suggested that the human osteosarcoma cells could synthesize and secrete angiogenesis factor with a molecular weight of 8000 to 10 000 u.

Key words

osteosarcoma angiogenesis factor 


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Copyright information

© Springer 2000

Authors and Affiliations

  • Wu Hua
    • 1
  • Wang Taiyi
    • 1
  • Deng Zhongdua
    • 2
  • Chen Duo’en
    • 2
  1. 1.Department of Orthopedics, Tongji HospitalTongji Medical UniversityWuhan
  2. 2.Department of Pathology, School of Basic Medical SciencesTongji Medical UniversityWuhan

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