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Journal of Tongji Medical University

, Volume 19, Issue 4, pp 319–322 | Cite as

Effects of hydrostatic pressure on cultured bovine trabecular meshwork cells

  • Jiang Fagang
  • Wei Houren
  • Lu Yuanshu
  • Zhou Yuanqing
  • Zhang Ying
Article
  • 17 Downloads

Summary

In order to explore the effects of pressure on trabecular meshwork cells, bovine trabecular meshwork cells were culturedin vitro and subjected to different levels of hydrostatic pressure. The cellular morphology, ultrastructure and phagocytosis were studied with inverted phase-contrast microscopy, light microscopy and transmission electron microscopy, etc. It was found that the cells subjected to 2. 0 kPa or 2. 67 kPa for 48 h had no remarkable difference as compared with the controls in terms of parameters observed. Those under 4. 0 kPa for 24 h showed slight changes in structure and a mild decrease in phagocytic function. The damage appeared more severe if the pressure was higher or lasted longer. From the above we conclude that trabecular meshwork cells can only bear pressure below a certain level. They may be destroyed structurally or impaired functionally by pressure over this level.

Key words

trabecular meshwork cells pressure glaucoma 

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Copyright information

© Springer 1999

Authors and Affiliations

  • Jiang Fagang
    • 1
  • Wei Houren
    • 1
  • Lu Yuanshu
    • 1
  • Zhou Yuanqing
    • 1
  • Zhang Ying
    • 1
  1. 1.Department of Ophthalmology, Xiehe HospitalTongji Medical UniversityWuhan

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