Biologia Plantarum

, Volume 32, Issue 4, pp 294–301 | Cite as

The relation between nitrogen deficiency and second leaf senescence in wheat plants

  • Alena Činčerová


Life span of the second leaf of wheat(Triticum aestivum L., cv. Grana) plants was studied from day 8 to day 50 of plant age in a variant with nitrogen (+N) and in a variant in which plant senescence was induced by the omission of nitrogen from the nutrient solution (−N). Seed protein was the sole source of nitrogen for these plants. Specific leaf mass (SLM) in the −N variant, and specific leaf area (SLA), the mass of fresh leaf, soluble protein content and total nitrogen content in the +N variant peaked by day 22 of plant age (that is by day 19 of leaf age). Dry matter content, leaf length and leaf area, and SLM in the +N variant peaked by day 29 of plant age (that is by day 26 of leaf age). The ontogeny of the second leaf in the variant with enhanced senescence was shorter by at least 14 days. Plants from this variant showed typical symptoms of N deficiency, that is yellowing of leaves, tip burn, and lack of tillering. However, the growth and biochemical characters studied did not indicate an earlier onset of the senescence of the second leaf of −N plants. Both +N and −N variants reached their peaks (with the exception of an earlier peak by day 12 in case of total nitrogen content in the −N variant) on the same day of leaf age. Thus the first part of the leaf life span from leaf growth initiation to full expansion was of the same length in both the control and N-def icient plants. The stage of the proper senescence of the second leaf of −N plants was very short; the leaf completely died away within 7 days after senescence onset.


Nutrient Solution Leaf Senescence Specific Leaf Area Total Nitrogen Content Soluble Protein Content 
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  1. Berger, M. G., Woo, K. C.,Wong, S. Ch., Fock, H. P.: Nitrogen metabolism in senescent flag leaves of wheat (T.aestivum L.) in the light. - Plant Physiol.78: 779–783, 1985.PubMedCrossRefGoogle Scholar
  2. Dvořák, M.: Influence of mineral nutritives on respiration ofCucurbita plant roots. - Acta Univ. Carol.-biol.1:65–86, 1960.Google Scholar
  3. Fleck, I., Fransi, A., Florensa, I., Rafales, M.: Relationship between inorganic phosphate and carbohydrate content in flag leaves ofTriticum aestivum L. cv. Kolibri. - J. Plant Physiol.126:283–291, 1986.Google Scholar
  4. Fleck, I., Fransi, A., Vidal, D.: Senescence patterns of several physiological and biochemical parameters of field grown flag leaves ofTriticum aestivum L. cv Kolibri. - J. Plant Physiol.123:327–338, 1986.Google Scholar
  5. Gregory, F. G.: Mineral nutrition of plants. - Annu. Rev. Biochem.6: 557–578, 1937.CrossRefGoogle Scholar
  6. Humphries, E. C.: Mineral components and ash analysis. - In:Paech, K., Tracey, M. V. (ed.): Moderne Methoden der Pflanzenanalyse. Vol. 1, Pp. 479–481. Springer-Veriag, Berlin 1956.Google Scholar
  7. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurements with Folin phenol reagent. - J. biol. Chem.193: 265–275, 1951.PubMedGoogle Scholar
  8. Mae, T., Ohira, K.: Relation between leaf age and nitrogen incorporation in the leaf of the rice plant(Oryza sativa). - Plant Cell Physiol.23:1019–1024, 1982.Google Scholar
  9. Mei, H. S., Thimann, K. V.: The relation between nitrogen deficiency and leaf senescence. - Physiol. Plant.62:157–161, 1984.CrossRefGoogle Scholar
  10. Mengel, K., Kirby, E. A.: Principles of Plant Nutrition. - International Potash Institute, Worblaufen-Bern 1979.Google Scholar
  11. de Molino, M. M., Ulloa, M., Martinez-Carasco, R., Pérez, P.: Characterization of grain yields and nitrogen level in relation to flag leaf senescence of wheat varieties. - Physiol. Plant.66: 503–508, 1986.CrossRefGoogle Scholar
  12. Simpson, F. J., Lambers, H., Dalung, M. J.: Translocation of nitrogen in a vegetative wheat plant.- Physiol. Plant.56:11–17, 1982.CrossRefGoogle Scholar
  13. Stoddart, J. C., Thomas, H.: Leaf senescence. - In:Boulter, D., Parthier, B. (ed.): Nucleic Acids and Proteins in Plants. I. Pp. 592–636, Springer-Verlag, Berlin-Heidelberg-New York 1982.Google Scholar
  14. Šesták, Z., Čatský, J.: Metody Studia Fotosyntetické Produkce Rostlin. [Methods of Studying Photosynthetic Production of Plants]. - Publ. House Czechosl. Acad. Sci., Praha 1966.Google Scholar
  15. Sesták, Z. (ed.): Photosynthesis during Leaf Development. - Academia, Praha 1985.Google Scholar
  16. Thomas, H., Stoddart, J. L.: Leaf senescence. - Annu. Rev. Plant Physiol.31: 83–111, 1980.CrossRefGoogle Scholar
  17. Ticha, I.: Ontogeny of leaf morphology and anatomy. - In:Šestak, Z. (ed.): Photosynthesis during Leaf Development. Pp. 16–50. Academia, Praha; Dr. W. Junk Publ., Dordrecht - Boston - Lancaster 1985.Google Scholar

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© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Alena Činčerová
    • 1
  1. 1.Department of Plant PhysiologyFaculty of Science, Charles UniversityPraha 2Czechoslovakia

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