Summary
Supernatants (SN) from 10 phytohemagglutinin (PHA)-stimulated human T cell clones (TCC), selected for their helper function on IgE synthesis, were found to provide IgE helper activity in atopic B cells showing low or undetectable spontaneousin vitro IgE synthesis. In contrast, SN from 5 PHA-stimulated TCC unable to provide helper function for IgE synthesis consistently failed to elicit IgE production. SN active on IgE synthesis contained high concentrations of interleukin-4 (IL-4), whereas inactive SN did not contain detectable amounts of IL-4. Moreover, the IgE helper activity of TCC SN was strongly inhibited by the addition of interferon-γ (IFN-γ) to B cell cultures. These data suggest that IL-4 may play a role in the induction ofin vitro human IgE synthesis, whereas IFN-γ displays an inhibitory effect.
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References
Coffman R. L., Carty J.: A T cell activity that enhances polyclonal IgE production and its inhibition by interferon-γ — J. Immunol.136, 949, 1986.
Del Prete G. F., Maggi E., Macchia D., Tiri A., Parronchi P., Ricci M., Romagnani S.: Human T cell clones can inducein vitro IgE synthesis in normal B cells regardless of alloantigen recognition or specificity for peculiar antigens — Europ. J. Immunol.16, 1509, 1986.
Lanzavecchia A., Parodi B.:In vitro stimulation of IgE production at a single precursor level by human alloreactive T-helper clones — Clin. exp. Immunol.55, 197, 1984.
Leung D. Y. M., Young M. C., Geha R. S.: Induction of IgG and IgE synthesis in normal B cells by autoreactive T cell clones — J. Immunol.136, 2851, 1986.
Moretta A., Pantaleo G., Moretta L., Cerottini J. C., Mingari M. C.: Direct demonstration of the clonogenic potential of every human peripheral blood T cell. Clonal analysis of HLA-DR expression and cytolytic activity — J. exp. Med.157, 743, 1983.
Nutman T. B., Volkman D. J., Hussain R., Fauci A. S., Ottesen E. A.: Filarial parasite-specific T cell lines: induction of IgE synthesis — J. Immunol.134, 1178, 1985.
Ricci M., Del Prete G. F., Maggi E., Lanzavecchia A., Sala P. G., Romagnani S.:In vitro synthesis of human IgE: reappraisal of a 5-year study — Int. Arch. Allergy77, 32, 1985.
Romagnani S., Damiani G., Giudizi M. G., Biagiotti R., Almerigogna F., Del Prete G. F., Maggi E., Bargellesi A., Ricci M.:In vitro production of IgE by human peripheral blood mononuclear cells. III. Demonstration of a circulating IgE-bearing cell involved in the spontaneous IgE biosynthesis — Clin. exp. Immunol.49, 176, 1982.
Romagnani S., Del Prete G. F., Maggi E., Bellesi G., Biti G., Rossi Ferrini P. L., Ricci M.: Abnormalities ofin vitro immunoglobulin synthesis by peripheral blood lymphocytes from untreated patients with Hodgkin’s disease — J. clin. Invest.71, 1375, 1983.
Romagnani S., Del Prete G. F., Maggi E., Ricci M.: Activation through CD3 molecule leads a number of human T cell clones to induce IgE synthesisin vitro by B cells from allergic and nonallergic individuals — J. Immunol.138, 1744, 1987.
Romagnani S., Giudizi M. G., Biagiotti R., Almerigogna F., Maggi E., Del Prete G. F., Ricci M.: Surface immunoglobulins are involved in the interaction of protein A with human B cells and in the triggering of B cell proliferation induced by protein A-containingStaphylococcus aureus — J. Immunol.127, 1307, 1981.
Umetsu D. T., Leung D. Y. M., Siraganian R., Jabara H. H., Geha R. S.: Differential requirements of B cells from normal and allergic subjects for the induction of IgE synthesis by an alloreactive T cell clone — J. exp. Med.162, 202, 1985.
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These studies were supported by grants from theMinistero della Pubblica Istruzione (contract № 12.02.01355) and from theConsiglio Nazionale delle Ricerche (CNR), Roma, Italy (contract № 87.01508).
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Maggi, E., Del Prete, G.F., Tiri, A. et al. Role of interleukin-4 in the induction of human IgE synthesis and its suppression by interferon-γ. La Ricerca Clin. Lab. 17, 363–367 (1987). https://doi.org/10.1007/BF02886920
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DOI: https://doi.org/10.1007/BF02886920