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Ricerca in clinica e in laboratorio

, Volume 17, Issue 4, pp 363–367 | Cite as

Role of interleukin-4 in the induction of human IgE synthesis and its suppression by interferon-γ

  • Enrico Maggi
  • Gian Franco Del Prete
  • Antonio Tiri
  • Donatella Macchia
  • Paola Parronchi
  • Mario Ricci
  • Sergio Romagnani
Original Contributions
  • 13 Downloads

Summary

Supernatants (SN) from 10 phytohemagglutinin (PHA)-stimulated human T cell clones (TCC), selected for their helper function on IgE synthesis, were found to provide IgE helper activity in atopic B cells showing low or undetectable spontaneousin vitro IgE synthesis. In contrast, SN from 5 PHA-stimulated TCC unable to provide helper function for IgE synthesis consistently failed to elicit IgE production. SN active on IgE synthesis contained high concentrations of interleukin-4 (IL-4), whereas inactive SN did not contain detectable amounts of IL-4. Moreover, the IgE helper activity of TCC SN was strongly inhibited by the addition of interferon-γ (IFN-γ) to B cell cultures. These data suggest that IL-4 may play a role in the induction ofin vitro human IgE synthesis, whereas IFN-γ displays an inhibitory effect.

Key-words

Interferon-γ Interleukin-4 In vitro IgE synthesis Phytohemagglutinin T cell clones 

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Copyright information

© Casa Editrice «Il Ponte» 1987

Authors and Affiliations

  • Enrico Maggi
    • 1
  • Gian Franco Del Prete
    • 1
  • Antonio Tiri
    • 1
  • Donatella Macchia
    • 1
  • Paola Parronchi
    • 1
  • Mario Ricci
    • 1
  • Sergio Romagnani
    • 1
  1. 1.Cattedra di Patologia Speciale Medica IV Clinica Medica IIIUniversità degli Studi di Firenze Policlinico di CareggiFirenzeItalia

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