Advertisement

Ricerca in clinica e in laboratorio

, Volume 17, Issue 4, pp 323–329 | Cite as

Serum IgA and IgG antibodies to α-gliadin: Comparison between two ELISA methods

  • Sandra Perticarari
  • Gianni Presani
  • Marina Trevisan
  • Adriano Savoini
  • Sabina Cauci
Original Contributions
  • 29 Downloads

Summary

We have developed two ELISA methods, i.e., enzyme immunoassay (EIA) and fluorescence immunoassay (FIA), for the semiquantitative detection of specific IgA and IgG antibodies directed against α-gliadin. The tests differ only for the enzyme substrate and, when optimized, could be used in large routine screening of celiac disease. Several serum samples from patients with celiac disease and gastrointestinal disorders as well as from control subjects were tested. Both methods gave good correlation with clinical data, were easily performed and had same specificity features, while FIA proved to be more sensitive.

Key-words

α-gliadin Celiac disease ELISA technique IgA and IgG antibodies 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bernardin J. E., Kasarda D. D., Mecham D. K.: Preparation and characterization of α-gliadin — J. biol. Chem.242, 445, 1967.PubMedGoogle Scholar
  2. 2.
    Burgin-Wolff A., Bertele R. M., Berger R., Gaze H., Harms H. K., Just M., Khanna S., Schürmann K., Signer E., Tomovic D.: A reliable screening test for childhood coeliac disease: fluorescent immunosorbent test for gliadin antibodies — J. Pediatr.102, 655, 1983.PubMedCrossRefGoogle Scholar
  3. 3.
    Carswell F., Ferguson A.: Plasma food antibodies during withdrawal and reintroduction of dietary gluten in coeliac disease — Arch. Dis. Childh.48, 583, 1973.PubMedCrossRefGoogle Scholar
  4. 4.
    Ciclitira P. J., Ellis H. J., Eyans D. J.: A solid-phase radioimmunoassay for measurement of circulating antibody titres to wheat gliadin and its subfractions in patients with adult coeliac disease — J. immunol. Meth.62, 231, 1983.CrossRefGoogle Scholar
  5. 5.
    Ciclitira P. J., Eyans D. J., Fagg N. L. K., Lennox E. S., Dowling R. H.: Clinical testing of gliadin fractions in coeliac patients — Clin. Sci.66, 357, 1984.PubMedGoogle Scholar
  6. 6.
    Finn R., Harvey M. M., Johnson P. M., Verbov J. L., Barnes R. M.: Serum IgG antibodies to gliadin and other dietary antigens in adults with atopic eczema — Clin. exp. Dermatol.10, 222, 1985.PubMedCrossRefGoogle Scholar
  7. 7.
    Howdle P. D., Ciclitira P. J., Simpson F. C., Losowsky M. S.: Are all gliadins toxic in coeliac disease? — Scand. J. Gastroenterol.19, 41, 1984.PubMedGoogle Scholar
  8. 8.
    Kilander A. F., Gillberg R. E., Kastrup W., Mobacken H., Nilsson L. Å.: Serum antibodies to gliadin and small intestinal morphology in dermatitis herpetiformis — Scand. J. Gastroenterol.20, 951, 1985.PubMedCrossRefGoogle Scholar
  9. 9.
    Lervenson S. D., Austin R. K., Dietler M. D., Kasarda D. D., Kagnoff M. F.: Specificity of anti-gliadin antibody in coeliac disease — Gastroenterology89, 1, 1983.Google Scholar
  10. 10.
    Lindberg T., Nilsson L. Å., Borulf S., Cavell B., Fällström S. P., Jansson U., Stenhammar L., Stintzing G.: Serum IgA and IgG gliadin antibodies and small intestinal mucosal damage in children — J. pediatr. gastroenterol. Nutr.4, 917, 1985.PubMedCrossRefGoogle Scholar
  11. 11.
    Scott H., Fausa O., Ek J., Brandtzaeg P.: Immune response patterns in coeliac disease. Serum antibodies to dietary antigens measured by ELISA — Clin. exp. Immunol.57, 25, 1984.PubMedGoogle Scholar
  12. 12.
    Signer E., Burgin-Wolff A., Berger R., Birbaumer A., Just M.: Antibodies to gliadin as a screening test for coeliac disease — Helv. paediatr. Acta34, 41, 1979.PubMedGoogle Scholar
  13. 13.
    Stenhammar L., Kilander A. F., Nilsson L. Å., Stromberg L., Tarkowski A.: Serum gliadin antibodies for detection and control of childhood coeliac disease — Acta paediatr. scand.73, 657, 1984.PubMedCrossRefGoogle Scholar
  14. 14.
    Unswort D. J., Leonard J. N., McMinn R. M. H., Swain A. F., Holborow E. J., Fry L.: Anti-gliadin antibodies and small intestinal mucosal damage in dermatitis herpetiformis — Brit. J. Dermatol.105, 653, 1981.CrossRefGoogle Scholar
  15. 15.
    Volta U., Lenzi M., Lazzari R., Cassani F., Collina A., Bianchi F. B., Pisi E.: Antibodies to gliadin detected by immunofluorescence and a micro-ELISA method: markers of active childhood and adult coeliac disease — Gut26, 667, 1985.PubMedCrossRefGoogle Scholar

Copyright information

© Casa Editrice «Il Ponte» 1987

Authors and Affiliations

  • Sandra Perticarari
    • 1
  • Gianni Presani
    • 1
  • Marina Trevisan
    • 2
  • Adriano Savoini
    • 3
  • Sabina Cauci
    • 4
  1. 1.Laboratorio di Analisi Biochimico-ClinicheIstituto per l’InfanziaTriesteItalia
  2. 2.Servizio di GastroenterologiaIstituto per l’InfanziaTrieste
  3. 3.Dipartimento di BiologiaUniversità degli Studi di TriesteItalia
  4. 4.Dipartimento di Biochimica, Biofisica e Chimica delle MacromolecoleUniversità degli Studi di TriesteItalia

Personalised recommendations