Ricerca in clinica e in laboratorio

, Volume 17, Issue 4, pp 323–329 | Cite as

Serum IgA and IgG antibodies to α-gliadin: Comparison between two ELISA methods

  • Sandra Perticarari
  • Gianni Presani
  • Marina Trevisan
  • Adriano Savoini
  • Sabina Cauci
Original Contributions


We have developed two ELISA methods, i.e., enzyme immunoassay (EIA) and fluorescence immunoassay (FIA), for the semiquantitative detection of specific IgA and IgG antibodies directed against α-gliadin. The tests differ only for the enzyme substrate and, when optimized, could be used in large routine screening of celiac disease. Several serum samples from patients with celiac disease and gastrointestinal disorders as well as from control subjects were tested. Both methods gave good correlation with clinical data, were easily performed and had same specificity features, while FIA proved to be more sensitive.


α-gliadin Celiac disease ELISA technique IgA and IgG antibodies 


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Copyright information

© Casa Editrice «Il Ponte» 1987

Authors and Affiliations

  • Sandra Perticarari
    • 1
  • Gianni Presani
    • 1
  • Marina Trevisan
    • 2
  • Adriano Savoini
    • 3
  • Sabina Cauci
    • 4
  1. 1.Laboratorio di Analisi Biochimico-ClinicheIstituto per l’InfanziaTriesteItalia
  2. 2.Servizio di GastroenterologiaIstituto per l’InfanziaTrieste
  3. 3.Dipartimento di BiologiaUniversità degli Studi di TriesteItalia
  4. 4.Dipartimento di Biochimica, Biofisica e Chimica delle MacromolecoleUniversità degli Studi di TriesteItalia

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