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Ricerca in clinica e in laboratorio

, Volume 7, Issue 4, pp 375–384 | Cite as

Study of the influence of haemodialysis on the β-methyl-digoxin levels in uraemic patients

  • Claudio Dotti
  • Pier Paolo Borgatti
  • Ugo Righi Riva
Original Contributions
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Summary

This study was undertaken to establish the plasma pharmacokinetics of β-methyl-digoxin (βmd) in 8 normal subjects (group 1) and 17 uraemic patients (group 2, not undergoing dialysis; group 3, undergoing dialysis). Blood samples were collected serially up to 5 hrs after an initial stimulus of 0.2 mg βmd i.v. βmd was determined both in serum and in dialysate by digoxin radioimmunoassay after having verified the following parameters: the immunological similarity between digoxin and βmd; the possibility of evaluating βmd in dialysate; the quantitative recovery of βmd both in serum and in dialysate. At 15 min after stimulus, the following βmd peaks occurred: 5.82, 3.70 and 6.00 ng/ml for groups 1, 2 and 3, respectively, with a significant difference (p<0.02) between groups 1 and 2 and between groups 2 and 3 (p<0.02) up to 1 h after stimulus. For each group the rapid, mean and slow βmd half-times (T 1/2) were calculated. The three T 1/2 values differing from each other but being related to the different initial βmd concentrations, the angular coefficients of the disappearance regression lines (taken as parameters related to the βmd disappearance rates, dr) were plotted against the βmd concentrations expressed as % of their highest value corresponding to the respective rapid T 1/2. From this approach, it can be deduced that the dr of group 1 is much higher than the dr of groups 2 and 3, which are similar. This finding, together with the consideration that we never found βmd in dialysate, permits the conclusion that the distribution rate of βmd to the tissues is significatively higher in normal than in uraemic patients and that it does not change during dialysis treatment.

Key-words

Dialysate Digoxin radioimmunoassay Serum disappearance rate Uraemia 

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Copyright information

© Casa Editrice «Il Ponte» 1977

Authors and Affiliations

  • Claudio Dotti
    • 1
  • Pier Paolo Borgatti
    • 2
  • Ugo Righi Riva
    • 3
  1. 1.1° Servizio di Radiologia (Sezione Radioisotopi)Arcispedale S. Maria NuovaReggio EmiliaItalia
  2. 2.Servizio di EmodialisiArcispedale S. Maria NuovaReggio Emilia
  3. 3.Servizio di CardiologiaArcispedale S. Maria NuovaReggio Emilia

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