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Journal of Tongji Medical University

, Volume 13, Issue 3, pp 138–142 | Cite as

Cryopreservation and culture of the human fetal brain tissues

  • Cai Rong-sheng
  • Xue De-lin
  • Jiang Xian-hui
Article

Summary

Human embryos after 3–4.5 months of gestation were obtained with abortion. The brain tissue of the bodies was scissored up to obtain 1–3 mm3 pieces, and 7% dymethyl sulfoxide (DMSO), as a cryoprotectant, was added, and then stored at −70°C for 1–30 days or at −196°C for 1–84 days. The survival rate of stored cells was 64%–88%. During 6 days of storage with neuron culture medium, the survival rate of cells at 4°C is over 50% each day, but, as time goes on, the count of the cells is getting less and less. The cells washed out DMSO after cryopreservation and the planting fresh cells can adhere to the wall of the culture bottle, grow, display various forms of neurons and gliacytes. From the above findings, it was suggested that: 1) The fetal human brain tissue, handled properly, can endure cryopreservation with7% DMSO as a cryoprotective agent; 2) The storage time was related insignificantly to the survival rate of the tissues stored; 3)It is available for a short proservation at 4°C and 4) It is possible to set up a bank of fetal human brain tissue.

Key words

fetal human brain tissue cryopreservation tissue culture freeze 

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Copyright information

© Springer 1993

Authors and Affiliations

  • Cai Rong-sheng
    • 1
  • Xue De-lin
    • 1
  • Jiang Xian-hui
    • 1
  1. 1.Department of Neurosurgery, Tongji HospitalTougji Medical UniversityWuhan

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