Journal of Tongji Medical University

, Volume 18, Issue 4, pp 221–224 | Cite as

Experimental study on the correlation of nitric oxide with portal hypertensive enteropathy

  • Yu Yunbo
  • Yin Chaoli
  • Yu Jinsheng
  • Liang Kuohuan


To explore the role of nitric oxide (NO) in the pathogenesis of portal hypertensive enteropathy (PHE), cirrhotic portal hypertension was induced in Wistar rats by subcutaneous administration of carbon tetrachloride. At the end of 12th week, NADPH-diaphorase staining was performed on ice frozen sections with the sample of fresh colonic tissues. NO synthase (NOS) activities and NOS mRNA expression of colonic tissues were investigated with chemoluminescence method and reverse transcription-polymerase chain reaction (RT-PCR), respectively. After NADPH-diaphorase histochemical staining, the computer image analysis and pairedt test showed that NOS staining intensities of submucosal vascular endothelial cells and nerve fibers were all significantly higher in PHE group than those in normal group (P<0.01 andP<0.05, respectively), but the intensities of superficial epithelial cells were significantly lower than those of controls (P<0.01). Chemoluminescence method demonstrated that general NOS activity of colonic mucosa was significantly higher in PHE group than that in control group (P<0.01). Moreover, the degree of iNOS activity increase was greater than that of cNOS (104 % vs 35 %). RT-PCR revealed that NOS mRNA expressions were dramatically higher in PHE group than those in control group (P<0. 01). The results suggested that NO, with its property of vasodilatation, may be involved in the pathogenesis of vascular lesions of PHE in cirrhotic rats, and may also has something to do with mucosal lesions of colon in PHE.

Key words

nitric oxide nitric oxide synthase portal hypertensive enteropathy 


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  1. 1.
    Viggiano T, Gostout C J. Portal hypertensive intestinal vasculopathy: A review of the clinical, endoscopic, and histopathologic features. Am J Gasroenterology, 1992, 87:944Google Scholar
  2. 2.
    Stark M E, Szurszewski J H. Role of nitric oxide in gastrointestinal and hepatic functions and diseases. Gastroenterology, 1992, 103:1928PubMedGoogle Scholar
  3. 3.
    Moncada S, Higgs A. The L-arginine-nitric oxide pathway. N Engl J Med, 1993, 329:2002PubMedCrossRefGoogle Scholar
  4. 4.
    1997, 26:147Google Scholar
  5. 5.
    Nunokawa Y, Ishida N, Tanaka S. Cloning of inducible nitric oxide synthase in rat vascular smooth muscle cells. Biochem Biophysiol Res Commun, 1993, 191:89CrossRefGoogle Scholar
  6. 6.
    NOS, AchE SP, 1997, 17:195Google Scholar
  7. 7.
    Mearin F, Mowrelle M, Guarner Fet al. Absence of nitric oxide synthases in gastroesophageal function of patients with achalasia. Gastroenterology, 1993, 104:550AGoogle Scholar
  8. 8.
    Vallance P, Moncada S. Hyperdynamic circulation in cirrhosis: a role for nitric oxide? Lancet, 1991, 337:776PubMedCrossRefGoogle Scholar
  9. 9.
    El-Newihi H M, Kanji V K, Mihas A A. Activity of gastric mucosal nitric oxide synthase in portal hypertensive gastropathy. Am J Gastroenterol, 1996, 91:535PubMedGoogle Scholar
  10. 10.
    Lopez-Talavera J C, Merrill W W, Groszmann R Jet al. Tumor necrosis factor a: a major contributor to hyperdynamic circulation in prehepatic portal hypertensive rats. Gastroenterology, 1995, 108:761PubMedCrossRefGoogle Scholar
  11. 11.
    Tanoue K, Sarfeh I J, Tarnawski Aet al. Overexpression of nitric oxide synthase gene and protein in portal hypertensive gastric mucosa. Gastroenterology, 1995, 108:1182AGoogle Scholar

Copyright information

© Springer 1998

Authors and Affiliations

  • Yu Yunbo
    • 1
  • Yin Chaoli
    • 1
  • Yu Jinsheng
    • 1
  • Liang Kuohuan
    • 1
  1. 1.Department of Internal Medicine, Tongji HospitalTongji Medical UniversityWuhan

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