Follicle-stimulating hormone (FSH) induced internalization of porcine FSH receptor in cultured porcine granulosa cells and chinese hamster ovary cells transfected with recombinant porcine FSH receptor cDNA

  • Zhu Changhong
  • Tian Hong
  • Xiong Zhongming
  • Xia Huizhu


In order to study the fate of human follicle-stimulating hormone (FSH) when hormone binds to its receptor, a quick biochemical method that can differentiate between the surface-bound and internalized hormone was used to determine the internalization induced by FSH in cultured both porcine granulosa cells and Chinese hamster ovary (CHO) cells expressing recombinant porcine FSH receptor. The results showed that FSH was slowly internalized, and the internalized radioactivity (acid resistant) reached a peak 10–12 h after addition of125I-hFSH. It was suggested that FSHR do not get internalized rapidly under physiological circumstances precisely because the appropriate sequences are absent.

Key words

follicle-stimulating hormone internalization granulosa cells 


  1. 1.
    LaBarbera A R, Ryan R J. Porcine granulosa cells in suspension culture. I. Follicle-stimulating hormone induction of human chorionic gonadotropin-binding sites on cells from small follicles. Endocrinology, 1981,108:1561PubMedCrossRefGoogle Scholar
  2. 2.
    Ferguson S S. Downey W E, Colapietro A Met al. Role of beta-arrestin in mediating agonist-promoted G proteincoupled receptor internalization. Science, 1996, 271:363PubMedCrossRefGoogle Scholar
  3. 3.
    Zhang J, Ferguson S S G, Barak L Set al. Dynamin and beta-arrestin reveal distinct mechanisms for G proteincoupled receptor internalization. J Biol Chem, 1996, 271:18302PubMedCrossRefGoogle Scholar
  4. 4.
    Ascoli M. Internalization and degradation of receptorbound human choriogonadotropin in Leydig tumor cells. J Biol Chem, 1982,257:13306PubMedGoogle Scholar
  5. 5.
    Knecht M, Ranta T, Katz M Set al. Regulation of adenylate cyclase activity by follicle-stimulating hormone and a gonadotropin-releasing hormone agonist in cultured rat granulosa cells. Endocrinology, 1983,112:1247PubMedGoogle Scholar
  6. 6.
    Sites C K, Patterson K, Jamison C Set al. Follicle-stimulating hormone (FSH) increases FSH receptor messenger ribonucleic acid while decreasing FSH binding in cultured porcine granulosa cells. Endocrinology, 1994,134: 411PubMedCrossRefGoogle Scholar
  7. 7.
    Fletcher P W, Reichert L E Jr. Cellular processing of follicle-stimulating hormone by Sertoli cells in serum-free culture. Mol Cell Endocrinol, 1984,34:39PubMedCrossRefGoogle Scholar
  8. 8.
    Shimizu A, Kawashima S. Kinetic study of internalization and degradation of131I-labeled follicle-stimulating hormone in mouse Sertoli cells and its relevance to other systems. J Biol Chem, 1989, 264:13632PubMedGoogle Scholar
  9. 9.
    Sanford J C, Batten B E. Endocytosis of follicle-stimulating hormone by ovarian granulosa cells: analysis of hormone processing and receptor dynamics. J Cell Physiol, 1989,138:154PubMedCrossRefGoogle Scholar
  10. 10.
    Trowbridge I S, Collawn J F, Hopkins C R. Signal-dependent membrane protein trafficking in the endocytic pathway. Annu Rev Cell Biol, 1993,9:129PubMedCrossRefGoogle Scholar
  11. 11.
    Rodriguez M C, Xie Y B, Wang Het al. Effects of truncations of the cytoplasmic tail of the luteinizing hormone/chorionic gonadotropin receptor on receptor-mediated hormone internalization. Mol Endocrinol, 1992,6:327PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2001

Authors and Affiliations

  • Zhu Changhong
    • 1
  • Tian Hong
    • 1
  • Xiong Zhongming
    • 1
  • Xia Huizhu
    • 1
  1. 1.Family Planning Research Institute, Center for Reproductive Medicine, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan

Personalised recommendations