Abstract
The protoplasts of Red Thorowax (Bupleurum scorzonerifolium) irradiated by ultraviolet light (UV) at an intensity of 260 μW/cm2 for 0, 1,2 and 3 min respectively were fused with that of grapevine (Vitia vinifera). The regenerated 19 clones, every one derived from a single fused cell, were identified as hybrids by phenotype, isozyme, chromosome and 5S rDNA spacer region analysis. The results reveal that all of them are somatic hybrids. 11 hybrid calli including asymmetric and symmetric products regenerated somatic embryos and young leaves after 5 months of culture, of which 4 hybrid cell lines derived from asymmetric fusion regenerated plants with roots after 8–10 months of culture. Inspection of chromosome showed that regeneration of whole plant was related to the decrease of chromosome number. Identification of 5S rDNA spacer region of the plants confirmed that they were interfamilial hybrid plants.
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References
Waara, S., Glimeliue, K., The potential of somatic hybridization in crop breeding,Euphytica., 1995, 85: 217.
Dudits, D., Maroy, D., Praznovzky, T.et al., Transfer of resistanre trait from carrot into tobarco by asymmetric somatic hyhridization: Regeneration of fertile plants,Proc. Natl. Acad. Sci. USA, 1987, 84: 8434.
Kisaka, H., Kameya, T., Production of somatic. hybrids between Daucuscarota L. andNicotiana tobacum, Theor. Appl. Genet., 1994, 88: 75.
Kisaka, H., Lee, H., Kisaka, M.et al., Production and analysis of asymmetric hybrid plants between monocotyledon (Oryza sativa L.) and dicotyledon (Daucus carota L.),Theor. Appl. Genet., 1994, 89: 365.
Kisaka, H., Kisaka, M., Kanno, A.et al., Production and analysis of plants that are somatic hybrids of barley (Hordeum vulgare L.) and carrot (Daucus carota L.),Theor. Appl. Genet., 1997, 94: 221.
Xia, G. M., Wang, H., Chen, H. M., Plant regeneration from intergeneric asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Russian wildrye (Psathyrostichys jurcea (Fisch) Neveski) and couch grass (Agropyron elongatum Host Neviski),Chinese Science Bulletin, 1996, 41(15): 1423.
Bao, X. Z., Fan, Z. G., Lin, C. F., Isolation and culture of grapevine pmtoplasts,J. Shandong Univ., 1993, 28(2): 248.
Xia, G. M., Li, Z. F., Chen, H. M.et al., Plant regeneration from protoplast ofBupleurum scorzonerifoliwn, Plant Cell Reports, 1992, 11: 155.
Xia, G. M., Chen, H. M., Plant regeneration from intergeneric somatic hybridization betweenTriticum aestivum andLeymus chinensis, Plant Science, 1996, 102: 197.
Rogers, D. C., Bendich, A. J., Extraction of DNA from plant tissue,Plant Molecular Biology Manua1, 1988, A6: 1.
Lu, B. Z., Yu, X. R., Guo, F. Y.et al., The embryogenesis from culturein vitro of filaments of grapevine,J. Fruit Science, 1995, (Supp.): 37.
Ko, H. L., Henry, R. J., Specific 5S ribosomal RNA primers for plant species identification in Admixture,Plant Molecular Biology Reporter, 1996, 14 (1): 33.
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Song, X., Xia, G., Zhou, A. et al. Hybrid plant regeneration from interfamilial somatic hybridization between grapevine (vitia vinifera L.) and Red Thorowax (Bupleurum scorzonerifolium willd). Chin. Sci. Bull. 44, 1878–1883 (1999). https://doi.org/10.1007/BF02886345
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DOI: https://doi.org/10.1007/BF02886345