Borf-1 protein identified as a transcriptionaltrans-activator of bovine foamy virus
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Bovine foamy virus (BFV), a member of the spumavirus subfamily of retroviruses, contains two open reading frames (ORF-1 and ORF-2) in addition to the genes coding forgag,pol andenv. Borf-1 protein, encoded by BFV ORF-1, is identified as a transcriptionaltrans-activator, which augments gene expression directed by the viral long terminal repeat (LTR). Further investigations in transient expression assays reveal that the Borf-1 responsive elements are located in the U3 domain of the LTR, upstream from position −140 (+ 1 represents the transcription initiation site), and the BFV RU5 region has an inhibitory effect in LTR-directed gene expression.
Keywordsbovine foamy virus (BFV)3026 China Strain transcriptionaltrans-activator transient expression assay
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