Abstract
Common carpCyprinus carpio genomic DNA repetitive sequence CR1 has been DIG-labeled and hybridizedin situ against chromosomes of red common carp (Cyprinus carpio L. Xingguo red var. ). It is found that the repetitive sequence CR1 is mainly localized at the centromeric regions of chromosomes of the red common carp. The application of the chromosomalin situ hybridization technique on fish and the relationship between CR1 repetitive sequence distribution and its function have been discussed.
Similar content being viewed by others
References
Clabby, C., Goswami, U., Flavin, F.et al., Cloning, characterization and chromosomal location of a satellite DNA from the Pacific oyster,Crassostrea gigas. Gene, 1996, 168: 205.
Gusella, J. F., Jones, C., Kao, F.et al., Genetic finestructure mapping in human chromosome 11 by the use of repetitive DNA sequences,Proc. Natl. Acad. Sci. USA., 1982, 79: 7804.
Looijenga, L. H. J., Smit, V. T. H. R. M., Wessels, J. W.et al., Localisation and polymorphism of a chromosome 12 specific α satellite DNA sequence,Cytogenet. Cell Genet., 1990, 53: 216.
Datta, U., Datta, P., Vandal, R. K., Cloning and characterization of a highly repetitive fish nucleotide sequence,Gene, 1988, 62: 331.
Moyer, S. P., Ma, D. P., Thomas, T. L.et al., Characterization of a highly repeated satellite DNA from the cyprinid fishNotropis lutrensis, Comp. Biochem. Physiol., 1988, 91B: 639.
He, L., Zhu, Z. Y., Cloning ofCyprinus carpio genomic DNA repetitive sequences,Annual Report of State Key Laboratory of Freshwater Ecology and Biotechnology (in Chinese), Institute of Hydrobiology, Chinese Academy of Sciences, 1989, 88/89: 165.
Wright, J. M., Nucleotide sequence, genomic organization and evolution of a major repetitive DNA family in tilapia (Oreochromis mossambicus/hornorum),Nucl. Acids Res., 1989, 17: 5071.
Denovan, E. M., Wright, J. M., A satellite DNA family from pollock (Pollachius virens),Gene, 1990, 87: 279–283.
Ekker, M., Fritz, A., Westerfield, M., Identification of two families of satellite-like repetitive DNA sequences from the zebra fish (Brachydanio rerio),Genornics, 1992, 13: 1169.
Garrido-Ramos, M. A., Jamilena, M., Lozano, R.et al., Cloning and characterization of a fish centromeric satellite DNA,Cytogenet. Cell Genet., 1994, 65: 233.
Yu, X. J., Zhou, T., Li, Y. C.et al., Chromosomes of Chinese Freshwater Fishes (in Chinese), Beijing: Science Press, 1989.
Sambrook, J., Fritsch, E. F., Maniatis, T.et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989.
Wang, S. W.,Techniques of Gene Diagnoses: Nonradioactive Practical Manual (in Chinese), Beijing: Beijing Medical University and Union Medical University Press, 1992.
Arnold, N., Seibl, R., Wienberg, J.et al., Nonradioactivein situ hybridization with digoxigenin-labeled DNA probes,Bwtechnic Histochemistry, 1990, 67(2): 59.
Phillips, R. B., Reed, K. M., Application of fluorescencein situ hybridization (FISH) techniques to fish genetics: a review,Aquaculture, 1996, 140: 197.
Pendas, A. M., Moran, P., Garcia-Vazquez, E., Ribosomal RSA genes are interspersed throughout a heterochromatic chromosome arm in Atlantic salmon,Cytogenet. Cell Genet., 1993, 63: 128.
Pendas, A. M., Moran, P., Freije, J. P.et al., Chromosomal mapping and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNA,Cytogenet. Cell Genet., 1994, 67: 31.
Pendas, A. M., Moran, P., Garcia-Vazquez, E., Organization and chromosomal location of the major histone cluster in brown trout, Atlantic salmon and rainbow trout,Chromosoma, 1994, 103: 147.
Reed, K. M., Phillips, R. B., Molecular characterization and cytogenetic analysis of highly repeated DNAs of lake trout,Snlvelinus namaycush., Chromosoma, 1995, 104: 242.
Reed, K. M., Phillips, R. B., Molecular cytogenetic analysis of the double-CMA, chromosome in lake trout,Salvelinus namaycush, Cytogenet. Cell Genet., 1995, 70: 104.
Haaf, T., Warburton, P. E., Willard, H. F., Integration of human α satellite DNA into simian chromosomes: centromere protein binding and disruption of normal chromosome segregation,Cell, 1992, 70: 681.
Jiang, Y. G., Liang, S. C., Chen, B. D.et a1., Biological effect of heterologous sperm on gynogenetic offspring inCarassius auratus gibelio, Acta Hydrobiologica Sinica (in Chinese), 1983, 8: 1.
Author information
Authors and Affiliations
About this article
Cite this article
Wang, Z., Zhu, Z. Chromosomalin situ hybridization ofCyprinus carpio genomic DNA repetitive sequence CR1. Chin.Sci.Bull. 44, 2242–2246 (1999). https://doi.org/10.1007/BF02885930
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02885930