Difference in expression of Bcl-2 and Bcl-xl genes in cisplatin-sensitive and cisplatin-resistant human in ovarian cancer cell lines
- 68 Downloads
To investigate the expression of Bcl-2 and Bcl-xl gene in sensitive (A2780) and drug-resistance (AD6) human ovarian cancer cell lines and explore the molecular mechanism of multidrug resistance. A2780 and AD6 were detected by using DNA gel electrophoresis, flow cytometry and RT-PCR. Our results showed that (1) “DNA ladder” was observed in A2780 and AD6 after cisplatin treatment; (2) after 3.0, 6.0, 9.9 μg/ml of cisplatin treatment, a significant difference was noted in the rate of apoptosis between in A2780 and AD6 (P<0.05); (3) Bcl-2 and Bcl-xl genes were overexpressed in AD6. After cisplatin treatment, the expression of Bcl-2 and Bcl-xl genes was down-regulated in A2780 and AD6. It is concluded that cisplatin could induce the apoptosis of ovarian cancer cells, and the over-expression of Bcl-2 and Bcl-xl genes may contribute to apoptotic inhibition and the development of multidrug-resistance of human ovarian cancer.
Key wordsovarian cancer apoptosis multidrug-resistance Bcl-2 and Bcl-xl cisplatin
Unable to display preview. Download preview PDF.
- 4.Alessandro Q, Laura P, Valeria Set al. Quantilation of bcl-2 oncogene in cultured lymphomal/leukemia cell lines and in primary leukemia B-cells by a highly sensitive RT-PCR method. Heamatologica, 1995, 80(6):495Google Scholar
- 6.Cure H, Bay J O, Plagne Ret al. High-dose chemotherapy in advanced epithelial ovarian cancer. Bull canner, 2001, 88(9):842Google Scholar