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Chinese Science Bulletin

, Volume 44, Issue 9, pp 796–799 | Cite as

Crystallization and preliminary crystallographic analyses of human insulin mutant B9(Ser→Asp) in different aggregation forms

  • Xinqi Liu
  • Lei Jin
  • Ying Zhang
  • Markussen Jan
  • Dacheng Wang
Notes
  • 49 Downloads

Abstract

B9(Ser→Asp) insulin, one of the fast-acting insulin mutants produced by protein engineering, has been crystallized. Three crystal forms have been obtained, which belong to orthorhombic, tetragonal and rhombohedral system, respectively. Each crystal form contains different aggregation units of the mutant, i.e. dimer or hexamer. The diffraction data of the former two have been collected beyond 0.2 nm resolution. The structural analyses and comparisons will provide some information about insulin ’ s self-association and the structural basis of the fast absorption produced from the mutation.

Keywords

insulin mutant B9Asp insulin crystallization crystallographic parameter 

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Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Xinqi Liu
    • 1
  • Lei Jin
    • 1
  • Ying Zhang
    • 1
  • Markussen Jan
    • 2
  • Dacheng Wang
    • 1
  1. 1.Institute of BiophysicsChinese Acndemy of SciencesBeijinsChina
  2. 2.Novo Research InstituteNovo AlleDenmark

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