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Chinese Science Bulletin

, Volume 44, Issue 2, pp 134–139 | Cite as

Synthesis and energy transfer of model conjugate of phycobilisome rods

  • Jiquan Zhao
  • Jingquan Zhao
  • Lijin Jiang
  • Jingmin Zhang
  • Fuli Zhao
  • Xiguang Zheng
  • Hezhou Wang
Notes

Abstract

The conjugate of R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) was synthesized through a heterobifunctional coupling reagent, N-succinimidyl 3-(2-pyridyldithio) propionate. The molar ratio of R-PE to C-PC was determined by absorption spectra, and the result was 2:1. The energy transfer phenomena were observed from steady-state fluorescence spectra. The calculated result showed that the energy transfer efficiency from R-PE to C-PC was 88%. The energy transfer kinetics was determined by picosecond time-resolved fluorescence spectra. The time constant of energy transfer from R-PE to C-PC was 80 ps, which was much longer than that in the rod of native phycobilisomes.

Keywords

R-phycoerythrin C-phycoeyanin rod model of phycobilisomes energy transfer time-resolved fluorescence spectra 

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Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Jiquan Zhao
    • 1
  • Jingquan Zhao
    • 1
  • Lijin Jiang
    • 1
  • Jingmin Zhang
    • 2
  • Fuli Zhao
    • 2
  • Xiguang Zheng
    • 2
  • Hezhou Wang
    • 2
  1. 1.Institute of Photographic ChemistryChinese Academy of SciencesBeijingChina
  2. 2.Institute of Laser and SpectraZhongshan UniversityGuangzhouChina

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