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A new method of recovering polyhydroxyalkanoate fromAzotobacter chroococcum

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Chinese Science Bulletin

Abstract

After polyhydroxyalkanoate (PHA) fermentation for 42 –48 h by theAzotobacter chroococcum G-3, the PHA content reached more than 75% of the dry weight. Biomass was isolated from culture by centrifugation and pretreated with freezing to release PHA pellet, then was treated with 10 g/L sodium dodecyl sulfate (SDS) for 15 min to effectively solubilize lipid and protein. Subsequently, it was further purified by digesting with 30% sodium hypochlorite (NaClO) for 3 min to remove peptidoglycan and non-PHA biomass. Finally, 98% PHA was obtained by diluting and rinsing with water, and the PHA recovered was suitable for processing.

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Authors and Affiliations

  1. Department of Biology, Northwest University Key Laboratory of Biotechnology of Shannxi, 710069, Xi’an, China

    Zhaolin Dong & Xuenan Sun

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  1. Zhaolin Dong
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  2. Xuenan Sun
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Dong, Z., Sun, X. A new method of recovering polyhydroxyalkanoate fromAzotobacter chroococcum . Chin.Sci.Bull. 45, 252–256 (2000). https://doi.org/10.1007/BF02884685

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  • DOI: https://doi.org/10.1007/BF02884685

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