Cloning and expression of a cDNA encoding ribosomal protein S4 from Rice (Oryza sativa)
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A cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous to eukaryotic 80s ribosomal protein subunit 4 (S4). Northern hybridization indicates that this gene expresses in all tissues analyzed although the expression level varies and it cannot be induced by mechanical wounding in leaves. Southern blot analysis demonstrates that this riceS4 gene is from a multigene family.
Keywordsribosomal protein S4 cDNA clone monocotyledonous plant tissue specificity (rice)
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- 10.Wool, I. G., Endo, Y., Chan, Y. L. et al., Studies of the structure, function, and evolution of mammalian ribosomes, in Ribosome Structure, Function, and Evolution (eds. Hill, W. E., Dahlberg, A., Garrett, R. A. et al.), Washington, DC: Am. Soc. Microbial., 1990, 203.Google Scholar
- 18.Xie, M., Chen, X., Qu, L. J. et al., Molecular cloning and sequence analysis of a gene encoding rice proteinase inhibitor, Acta Botanica Sinica, 1996, 38(6): 444.Google Scholar