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Chinese Science Bulletin

, Volume 43, Issue 11, pp 936–940 | Cite as

Novel gene expressed during early embryogenesis of zebrafish identified by mRNA differential display

  • Li Shuhong
  • Guo Shaodong
  • Sun Fangzhen
Bulletin
  • 12 Downloads

Abstract

Following the revelation of the molecular mechanism of morphogenesis in fruitfly, research on the molecular mechanism of morphogenesis in vertebrate becomes the focus of developmental biology. The isolation of genes controlling the embryogenesis of zebrafish, a vertebrate model animal, is considered as an initial step toward investigating this iasue. There are several approaches that can be used to isolate developmental genes, each of which is suited to a particular situation. In this note, mRNA differential display was utilized to demonstrate the mRNA differences among zebrafish embryos at 4, 5 and 6 h post fertilization (28.5°C, corresponding to oblong, dome and shield stages, respectively, called blastula, gastrula and neumla in this note). One cDNA tag that was specific to embryos at neurula stage was cloned and sequenced. After sequence comparison in Genbank, we found that this cDNA tag represents a novel gene. The expression of this gene in the developing zebrafish embryos was examined by whole mountin situ hybridization. The hybridization results confirmed that this gene was specifically expressed in zebrafish neurula embryos.

Keywords

mRNA differential display zebrafish morphogenesis gene 

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References

  1. 1.
    Concordet, J. P., Ingham, P., Catch of the decade,Nature, 1994, 369: 19.PubMedCrossRefGoogle Scholar
  2. 2.
    Barinaga, M.. Imking to development’s future,Science, 1994, 266: 561.PubMedCrossRefGoogle Scholar
  3. 3.
    Li, S. H., Zebrafish as a vertebrate model for development studies,Dev. Rep. Biol., 1997, 6(1): 65.Google Scholar
  4. 4.
    Roush, W., Zebrafish embryology builds better model vertebrate,Science, 1996, 272: 1103.PubMedCrossRefGoogle Scholar
  5. 5.
    Holder, N., McMahon, N., Genes from zebrafish screens,Nature, 1996, 384: 515.PubMedCrossRefGoogle Scholar
  6. 6.
    St. John, T. P., Davis, R. W., Isolation of galactose-inducible DNA sequences from Saecharomyces cerevisiae by differential plaque filter hybridization.Cell, 1979, 16:443.PubMedCrossRefGoogle Scholar
  7. 7.
    Zimmerman, C. R., Orr, W. C., Leclerc, R. F.et al., Molecular cloning and selection of genes regulated in Aspergillus development.Cell, 1980, 21: 709.CrossRefGoogle Scholar
  8. 8.
    Liang, P., Pardee, A. B., Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction,Science, 1992, 257: 967.PubMedCrossRefGoogle Scholar
  9. 9.
    Conway, G., A novel gene expressed during zebrafish gastrulation identified by differential RNA display,Mech. Dev., 1995, 52: 383.PubMedCrossRefGoogle Scholar
  10. 10.
    Li, S. H., Han, W., Ma, H. F., Application of mRNA differential display to the identification of development related genes,Dev. Rep. Biol., 1997, 6(2): 67.Google Scholar
  11. 11.
    Bauer, D., Warthoe, P.. Rohde, M.et al., Detection and differential display of expressed genes by DDRT-PCR,PCR Methods and Applications, 1994, 4: S97.PubMedGoogle Scholar
  12. 12.
    Westerfield, M.,The Zebrafish Book, Eugene: University of Oregon Press, 1994.Google Scholar
  13. 13.
    Postlethwait, J. H., Johnson, S. L., Midson, C. N.et al., A genetic linkage map for the zebrafish,Science, 1994, 264: 699.PubMedCrossRefGoogle Scholar
  14. 14.
    Knapik, E. M., Goodman, A., Atkinson, O. S.et al., A reference cross DNA panel for the zebrafish (Danio rerio) anchored with simple sequence length polymorphisms,Development, 1996, 123:451.PubMedGoogle Scholar
  15. 15.
    Dawid, I. B., Intercellular signaling and gene regulation during early embryogenesis ofXenopus laevis, J. Bwl. Chem., 1994, 269: 6259.Google Scholar
  16. 16.
    Kane, D. A., Kimmel, C. B., The zebrafish midblastula transition,Development, 1993, 119: 447.PubMedGoogle Scholar

Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Li Shuhong
    • 1
  • Guo Shaodong
    • 1
  • Sun Fangzhen
    • 1
  1. 1.Institute of Developmental BiologyBeijingChina

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