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Chinese Science Bulletin

, Volume 43, Issue 4, pp 292–295 | Cite as

An alternative splice variant of human thioredoxin

  • Wang Ying
  • Wang Yugang
  • Zhang Yingmei
  • Yuan Yong
  • Ma Dalong
Bulletin

Abstract

An alternative splice variant of human thioredoxin (hTRX) has been identified by using reverse transcription-polymerase chain reaction, cloning and expressing of hTRX-encoding gene, and DNA sequencing. This variant contains hTRX exons 1, 2, 4 and 5. The entire protcin encoding region of this variant, named hTRX63, is identical to hTRX except for the omission of exon 3. Human TRX and TRXδ3 cDNA has been expressed inE. coli. The catalytic activity of rhTRXδ3 on DTT-dependent insulin reduction is lower than that of rhTRX, although hTRXδ3 still contains the active site.

Keywords

human thioredoxin alternative splicing variant isofom E.coli 

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Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Wang Ying
    • 1
  • Wang Yugang
    • 1
  • Zhang Yingmei
    • 1
  • Yuan Yong
    • 1
  • Ma Dalong
    • 1
  1. 1.Department of ImmunologyBeijing Mcdical UniversityBeijingChina

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