Folia Microbiologica

, Volume 14, Issue 6, pp 554–556 | Cite as

Induction of respiration-deficient mutants by ultraviolet radiation in a synchronous yeast cell culture

  • V. Kováčová
  • D. Vlček
  • E. Miadoková


Haploid cells ofSaccharomyces cerevisiae in a synchronous culture were exposed in different phases of division to UV radiation lethal for 98% of the cells and the occurrence of respiration-deficient mutants among the survivors was determined. Comparison of the various phases showed significantly higher occurrence of these mutants at the onset of budding. It was concluded that the mitochondrial DNA also replicates synchronously in a synchronous yeast cell culture, but in a different phase from the nuclear DNA.


Haploid Cell Saccharo Synchronous Culture Overlay Technique Sectored Coloni 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ephrussi, B., Hottinguer, H., Chimenes, A. M.:Action de l'acriflavine sur les levures. I. La mutation ‘petite colonie’. Ann. Inst. Pasteur 76: 351, 1949.Google Scholar
  2. Hilz, H., Eckstein, H.:Teilungssynchronisierte Hefezellen. I. Unterschiedliche Wirkungen von Röntgenstrahlen und cytostatischen Verbindungen auf Stoffwechsel und Zellteilung. Biochem. Z. 340: 351, 1964.PubMedGoogle Scholar
  3. Kováčová, V., Vlček, D., Miadoková, E.:Synchronization of a yeast cell culture and induction of respiration-deficient mutants by UV radiation. (In Slovak) Acta facult. rer. nat. Univ. Commenianae, 1969 (in press).Google Scholar
  4. Mounolou, J. C., Jakob, H., Slonimski, P. P.:Mitochondrial DNA from yeast “petite” mutants: Specific changes of buyonant density corresponding to different cytoplasmic mutations. Biochem. Biophys. Res. Commun. 24: 218, 1966.PubMedCrossRefGoogle Scholar
  5. Nagai, S., Yanagishima, N., Nagai, H.:Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms. Bacteriol. Rev. 25: 404, 1961.PubMedGoogle Scholar
  6. Ogur, M., St. John, R., Nagai, S.:Tetrazolium overlay technique for population studies of respiratory deficiency in yeast. Science 125: 928, 1957.PubMedCrossRefGoogle Scholar
  7. Pittman, D. D.:Ultraviolet induction of respiration-deficient variants of Saccharomyces and their stability during vegetative growth. Cytologia 24: 315, 1959.Google Scholar
  8. Roodyn, D. B., Wilkie, D.:The Biogenesis of Mitochondria. p. 66 Methuen, London 1968.Google Scholar
  9. Samoylova, A. K.:Effect of ultraviolet radiation on the cell. (In Russian) Acad. Sci. USSR, Moscow, 1967.Google Scholar
  10. Williamson, D. H., Scopes, A. W.:The distribution of nucleic acids and protein between different sized yeast cells. Exptl. Cell Res. 24: 236, 1961.CrossRefGoogle Scholar
  11. Williamson, D. H., Scopes, A. W.:Rapid method for synchronizing division in the yeast Saccharomyces cerevisiae. Nature 193: 256, 1962.CrossRefGoogle Scholar
  12. Wintersberger, E.:Occurrence of DNA-polymerase in isolated yeast mitochondria. Biochem. biophys. Res. Commun. 25: 1, 1966.PubMedCrossRefGoogle Scholar
  13. Yotsuyanagi, Y.:Études sur le chondriome de la levare. I, II. J. Ultrastr. Res. 7: 121, 1962.CrossRefGoogle Scholar

Copyright information

© Academia, nakladatelství Československé akademie věd 1969

Authors and Affiliations

  • V. Kováčová
    • 1
  • D. Vlček
    • 1
  • E. Miadoková
    • 1
  1. 1.Department of Genetics, Faculty of ScienceKomensky UniversityBratislava

Personalised recommendations