Induction of respiration-deficient mutants by ultraviolet radiation in a synchronous yeast cell culture
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Haploid cells ofSaccharomyces cerevisiae in a synchronous culture were exposed in different phases of division to UV radiation lethal for 98% of the cells and the occurrence of respiration-deficient mutants among the survivors was determined. Comparison of the various phases showed significantly higher occurrence of these mutants at the onset of budding. It was concluded that the mitochondrial DNA also replicates synchronously in a synchronous yeast cell culture, but in a different phase from the nuclear DNA.
KeywordsHaploid Cell Saccharo Synchronous Culture Overlay Technique Sectored Coloni
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- Ephrussi, B., Hottinguer, H., Chimenes, A. M.:Action de l'acriflavine sur les levures. I. La mutation ‘petite colonie’. Ann. Inst. Pasteur 76: 351, 1949.Google Scholar
- Kováčová, V., Vlček, D., Miadoková, E.:Synchronization of a yeast cell culture and induction of respiration-deficient mutants by UV radiation. (In Slovak) Acta facult. rer. nat. Univ. Commenianae, 1969 (in press).Google Scholar
- Pittman, D. D.:Ultraviolet induction of respiration-deficient variants of Saccharomyces and their stability during vegetative growth. Cytologia 24: 315, 1959.Google Scholar
- Roodyn, D. B., Wilkie, D.:The Biogenesis of Mitochondria. p. 66 Methuen, London 1968.Google Scholar
- Samoylova, A. K.:Effect of ultraviolet radiation on the cell. (In Russian) Acad. Sci. USSR, Moscow, 1967.Google Scholar