Folia Microbiologica

, Volume 36, Issue 5, pp 423–428 | Cite as

Extracellular proteinase fromEnterococcus faecalis subsp.liquefaciens

I. Growth and extracellular proteinase production under different culture conditions
  • G. D. García de Fernando
  • P. E. Hernández
  • J. Burgos
  • B. Sanz
  • J. A. Ordóňez


Growth and extracellular proteinase production byEnterococcus faecalis subsp.liquefaciens was studied on several culture media and under different incubation conditions. The organisms grew well and developed extracellular proteinase activity on proteinaceous media, but when it grew on Collins basal medium (lacking of protein), growth was poor and proteinase activity was not detected. The activation energy for growth was estimated to be 116 kJ/mol, the optimum being at 37°C. Proteinase production was not affected by temperature in the range studied (7–45°C). Growth rate was not affected by aeration although a higher amount of microorganisms was observed on shaking the culture during incubation. Likewise, extracellular proteolytic activity was about twice higher in cultures shaken at 2.3 or 3.3 Hz than in those shaken at 0 or 1.3 Hz.


Proteolytic Activity Extracellular Proteinase Proteinase Production Cheddar Cheese bRCA 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1991

Authors and Affiliations

  • G. D. García de Fernando
    • 1
  • P. E. Hernández
    • 1
  • J. Burgos
    • 2
  • B. Sanz
    • 1
  • J. A. Ordóňez
    • 1
  1. 1.Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de VeterinariaUniversidad ComplutenseMadridSpain
  2. 2.Departamento de Producción Animaly Ciencia de los Alimentos, Facultad de VeterinariaUniversidad de ZaragozaZaragozaSpain

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