Chinese Science Bulletin

, Volume 43, Issue 20, pp 1732–1736 | Cite as

Callus formation in relation to changes of the membrane Ca2+ in the graft unions ofGinkgo biloba L.

  • Xiong Yang
  • Zhengli Li


The relationship between the callus formation and intracellular Ca2+ at the early stage of development of graft unions inGinkgo biloba L. was investigated. On the second day after grafting, part of the undamaged living cells at the graft interface of the stock and scion in the regions of the cortex and pith began to dedifferentiate or divide. 8 d after grafting, a great number of callus cells were produced from the cortex and phloem, while callus bridges linking the stock with scion formed between the callus cells of the graft partners from the cortex. A membrane Ca2+ probe, chlortetracycline (CTC), was used to locate the intracellular Ca2+ distribution and it was found that the cortical and pith parenchyma cells were the first to show remarkably increased CTC-Ca2+ fluorescence, suggesting that the cortical and other parenchyma cells play a leading role at the early stage of development of graft unions by earlier dedifferentiation and more rapid production of callus cells.


callus formation membrane Ca2+ graft union Ginkgo biloba 


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Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Xiong Yang
    • 1
  • Zhengli Li
    • 1
  1. 1.College of Life SciencesPeking UniversityBeijingChina

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