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Chinese Science Bulletin

, Volume 43, Issue 6, pp 490–493 | Cite as

Effect of lanthanum ions on tRNAphe structure: Imino proton NMR spectroscopy

  • Huamin Tu
  • Yijie Wu
  • Yansheng Yang
  • Menglian Gong
  • Hongjie Zhang
Bulletin
  • 15 Downloads

Abstract

The effect of lanthanum ions on the structural and conformational change of yeast tRNAphe was studied by1H NMR. The results suggest that the tertiary base pair (G-15)(C-48), which was located in the terminal in the augmented dihydrouridine helix (D-helix), was markedly affected by adding La3+ and shifted 0.33 downfield. Based pair (U-8)(A-14), which is associated with a tertiary interaction, links the base of the acceptor stem to the D-stem and anchors the elbow of the L structure, shifted 0.20 upfield. Another imino proton that may be affected by La3+ in tRNAphe is the tertiary base pair (G-19)(C-56). The assignment of this resonance is tentative since it is located in the region of highly overlapping resonances between 12.6 and 12.2. This base pair helps to anchor the D-loop to the TΨC loop.

Keywords

lanthanum ion transfer ribonucleic acid NMR 

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References

  1. 1.
    Reid, S. S., Cowan, J. A., Biostructural chemistry of magnesium ion: Characterization of the weak binding sites on tRNAPhe (yeast). Implications for conformational change and activity,Biochemistry, 1990, 29: 6025.PubMedCrossRefGoogle Scholar
  2. 2.
    Choi, B. S., Redfield, A. G., Proton exchange and basepair kinetics of yeast tRNAPhe and tRNAPhe,J. Biochem., 1995, 117: 515.PubMedCrossRefGoogle Scholar
  3. 3.
    Meng, J. X., Gong, M. L., Yang, Y. S.et al., Determination of association constants of Ce3+-tRNA complexes with Ce3+ fluorescence, inAbstracts for 15th Eurasia Conference on Chemical Sciences, Guangzhou: Zhongshan University Press, 1996, 513.Google Scholar
  4. 4.
    Ciesiobka, J., Marciniec, T., Krzyzosiak, W. J., Probing the environment of lanthanide binding sites in yeast tRNAPhe by specific metal-ion-promoted cleavages,Ear. J. Biochem., 1989, 182: 445.CrossRefGoogle Scholar
  5. 5.
    Brown, R. S., Dewan, J. C., Klug, A., Crystallographic and biochemical investigation of the lead(II)-catalyzed hydrolysis of yeast phenylalanine tRNA,Biochemistry, 1985, 24: 4785.PubMedCrossRefGoogle Scholar
  6. 6.
    Arter, D. B., Schmidt, P. G., Ring current shielding effects in nucleic acids res,Nucl. Acids. Res., 1976, 3: 1437.PubMedGoogle Scholar
  7. 7.
    Sklenar, V., Bax, A., Spin-echo water suppression for the generation of pure-phase two-dimensional NMR spectra,J. Magn. Reson., 1987, 74: 469.Google Scholar
  8. 8.
    Yue, D. X., Kintanar, A., Horowitz, J., Nucleoside modifications stabilize Mg2+ binding inE. coli tRNAVal, An imino proton NMR investigation,Biochemistry, 1994, 33: 8905.PubMedCrossRefGoogle Scholar
  9. 9.
    Roy, S., Redfield, A. G., Assignment of imino proton spectra of yeast phenylalanine transfer ribonucleic acid,Biochemistry, 1983, 22: 1386.PubMedCrossRefGoogle Scholar
  10. 10.
    Heerschap, A., Haasnoot, C. A. G., Hilbers, C. W., Nuclear magnetic resonance studies on yeast tRNAphe, I. Assignment of the imino proton resonances of the acceptor and D stem by means of nuclear Overhauser effect experiments at 500 MHz,Nucleic Acids Res, 1982, 10: 6981.PubMedCrossRefGoogle Scholar
  11. 11.
    Heerschap, A., Haasnoot, C. A. G., Hilbers, C. W., Nuclear magnetic resonance studies on yeast tRNAphe, II. Assignment of the imino proton resonances of the anticodon and T stem by means of nuclear Overhauser effect experiments at 500 MHz,Nucleic Acids Res., 1983, 11: 4483.PubMedCrossRefGoogle Scholar
  12. 12.
    Heerschap, A., Haasnoot, C. A. G., Hilbers, C. W., Nuclear magnetic resonance studies on yeast tRNAphe III. Assignment of the imino proton resonances of the tertiary structure by means of nuclear Overhauser effect experiments at 500 MHz,Nucleic Acids Res., 1983, 11: 4501.PubMedCrossRefGoogle Scholar
  13. 13.
    Heerschap, A., Mellema, J. R., Janssen, H. J. G. M.et al., Imino proton resonances of yeast tRNAphe studied by twodimensional nuclear Overhauser enhancement spectroscopy,Eur. J. Biochem., 1985, 149; 649.PubMedCrossRefGoogle Scholar
  14. 14.
    Wong, Y. P., Reid, B. R., Kearns, D. R., Conformation of charged and uncharged phenylalanine tRNA,Proc. Natl. Acad. Sci. USA, 1973, 70: 2193.PubMedCrossRefGoogle Scholar
  15. 15.
    Flanagan, J. M., Jacobson, K. B., Effect of zinc ions on tRNA structure: Imino proton NMR spectroscopy,Biochemistry, 1988, 27: 5778.PubMedCrossRefGoogle Scholar
  16. 16.
    Choi, B. S., Redfield, A. G., Nuclear magnetic resonance observation of the triple interaction between A9 and AU12 in yeast tRNAphe,Nucleic Acids Res., 1985, 13: 5249.PubMedCrossRefGoogle Scholar
  17. 17.
    Hingerty, B., Brown, R. S., Jack, A., Further refinement of the structure of yeast tRNAphe,J. Mol. Biol., 1978, 124: 523.PubMedCrossRefGoogle Scholar

Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Huamin Tu
    • 1
  • Yijie Wu
    • 2
  • Yansheng Yang
    • 1
  • Menglian Gong
    • 1
  • Hongjie Zhang
    • 2
  1. 1.Department of ChemistryZhongshan UniversityGuangzhouChina
  2. 2.Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchunChina

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