FSHB subunit gene is associated with major gene controlling litter size in commercial pig breeds
- 53 Downloads
An insertion fragment in porcine FSHβ subunit gene was cloned by PCR. Sequencing data show that the insertion is a retroposon of 292 bp siting in intron I at the site between + 809 and +810 base. Based on these results, a PCR programme was created to genotype animal individuals in different pig breeds at FSW locus and polymorphism of FSHP gene was analyzed. With the combination of genotype and litter size of sows, it was demonstrated that FSHβ locus is closely associated with major gene controlling litter size in commercial pig breeds, such as Yorkshire, Landrace, Durco. Averagely the AA sows give more 1.5 piglets than BB sows do per litter.
KeywordsFSHβ subunit gene litter size major gene candidate gene approach
Unable to display preview. Download preview PDF.
- 1.Li, N., Zhao, Y.F., Zhang, F.J. et al., Research on porcine litter size by linkage analysis,The Symposium of the 8th National Conference on Animal Genetics and Breeding (in Chinese), Beijing: China Agricultural Technology Press, 1995, 32–41.Google Scholar
- 2.Sambrook, J., Fritsch, E.F., Maniatis, T.,Molecular Cloning, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989.Google Scholar
- 4.Zhao, Y.F., Li, N., Feng, J.D. et al., PCR amplification of structural region of FSHβ subunit gene,The Symposium of the 8th National Conference on Animal Genetics and Breeding (in Chinese), Beijing: China Agricultural Technology Press, 1995, 51–53.Google Scholar
- 8.Van der Steen, H.A.M., Southwad, O.I., Vires, A.G. et al., Evidence of a new genetic marker for litter size in Meishan synthetic pigs,Proceedings of International Conference on Animal Biotechnology, Beijing: International Academic Publishers, 1997, 17–20.Google Scholar