Skip to main content
Log in

NodD binds to target DNA in isologous octamer

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

NodD, the major regulatory protein of nodulation, was partially purified from Rhizobium leguminosarum 8401(pIJ1518), and its binding sequences within nodF promoter of R. l. bv. viciae were determined by DNase I footprinting. A series of techniques based on gel retardation were used to analyze the NodD-target DNA interaction, showing that NodD binds to target DNA in isologous octamer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Hong, G. F., Burn, J. E., Johnston, A. W. B., Evidence that DNA involved in the expression of nodulation (nod) genes inRhizobium binds to the product of the regulatory genenodD, Nucl. Acids. Res., 1987, 15: 9677.

    Article  PubMed  CAS  Google Scholar 

  2. Fisher, R. F., Long, S. R., DNA footprint analysis of the transcriptional activator proteins NodD1 and NodD3 on induciblenod gene promoters,J. Bacteriol., 1989, 171: 5492.

    PubMed  CAS  Google Scholar 

  3. Fisher, R. F., Long, S. R., Interaction of NodD at thenod box: NodD binds to two distinct sites on the same face of the helix and induces a bend in the DNA,J. Mol. Biol., 1993, 233: 336.

    Article  PubMed  CAS  Google Scholar 

  4. Lu, Y. Y., Xu, Y. C., Hong, G. F., Determination of DNA sequences protected by NodD proteins,Chin. Sci. Bull., 1994, 39: 1843.

    CAS  Google Scholar 

  5. Rostas, K., Kondorosi, E., Horvath, B. et al., Conservation of extended promoter regions of nodulation genes inRhizobium, Proc. Natl. Acad. Sci. USA, 1986, 83: 1757.

    Article  PubMed  CAS  Google Scholar 

  6. Wang, S. P., Stacey, G., Studies of theBradyrhizobium japonicum nodD1 promoter: a repeated structure for thenod box,J. Bacteriol., 1991, 173: 3356.

    PubMed  CAS  Google Scholar 

  7. Goethals, K., Van Montagu, M., Holsters, M., Conserved motifs in a divergentnod box ofAzorhizobium caulinodans ORS571 reveal a common structure in promoters regulated by LysR-type proteins,Proc. Natl. Acad. Sci. USA, 1992, 89: 1616.

    Article  Google Scholar 

  8. Fried, M., Crothers, D. M., Equilibria and kinetics oflac repressor-operator interactions by polyacrylamide gel electrophoresis,Nucl. Acids Res., 1981, 9: 6505.

    Article  PubMed  CAS  Google Scholar 

  9. Galas, D. J., Schmitz, A., DNase footprinting: a simple method for the detection of protein-DNA binding specificity,Nucl. Acids. Res., 1978, 5: 3157.

    Article  PubMed  CAS  Google Scholar 

  10. Bayer, E. A., Wilchek, M., Protein biotinylation,Methods Enzymol., 1990, 184: 138.

    Article  PubMed  CAS  Google Scholar 

  11. Straney, D. C., Crothers, D. M., Intermediates in transcription initiation from theE. coli lac UV5 promoter,Cell, 1985, 43: 449.

    Article  PubMed  CAS  Google Scholar 

  12. Orchard, K., May, G. E., An EMSA-based method for determining the molecular weight of a protein-DNA complex,Nucl. Acids Res., 1993, 21: 3335.

    Article  PubMed  CAS  Google Scholar 

  13. Ogawa, J., Long, S. R., TheRhizobium meliloti groELc locus is required for regulation of earlynod genes by the transcription activator NodD,Genes Dev., 1995, 9: 714.

    Article  PubMed  CAS  Google Scholar 

  14. Baeuerle, P. A., Baltimore, D., Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kB transcription factor,Cell, 1988, 53: 211.

    Article  PubMed  CAS  Google Scholar 

  15. Govezensky, D. T., Greener, T., Segal, G. et al., Involvement of GroEL innif gene regulation and nitrogenase assembly,J. Bacteriol., 1991, 173: 6339.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Project supported by Pan-Deng Plan. The first two authors contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, S., Lü, H. & Hong, G. NodD binds to target DNA in isologous octamer. Sci. China Ser. C.-Life Sci. 41, 592–599 (1998). https://doi.org/10.1007/BF02882900

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02882900

Keywords

Navigation