Science in China Series C: Life Sciences

, Volume 41, Issue 5, pp 525–529 | Cite as

Molecular cloning, sequence analysis and expression studies of a novelGAmyb homologous gene,hvmyb

  • Shiyun Ling
  • Fang Qi
  • Yuxian Zhu


Using a knownGAmyb gene as the probe, two fully identical clones were isolated from a barley aleurone cDNA library. Sequence analysis showed that their 5′ termini are highly homoIogous to the 3′ termini ofGAmyb (97%) and their 3′ termini share no significant homology with any myb genes. Therefore, the deduced protein may hold intact putativeGAmyb activation domain but lack the normal DNA-binding domain. Northern blot reveals thathumyb expression in barley aleurone layers is strongly up-regulated by gibberellin (GA) and down-regulated by abscisic acid (APIA). The tissue-and developmental-stage-specificity ofhvmyb was also found, which was only expressed in barley aleurone cells and dropped to non-detectable level soon after germination.


barley aleurone GAmyb GA ABA 


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Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Shiyun Ling
    • 1
  • Fang Qi
    • 1
  • Yuxian Zhu
    • 1
  1. 1.State Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life SciencesPeking UniversityBeijingChina

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