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Chinese Science Bulletin

, Volume 42, Issue 15, pp 1300–1303 | Cite as

Human antibodies from a single-chain Fv fusion phage library

  • Xiyun Yan
  • Bo Tian
Bulletin
  • 16 Downloads

Keywords

human scFv phage antibody library specificity 

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References

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    Ward, E. S., Gussow, D., Griffiths, A. D., Binding activities of a repertoire of single immunoglobulin variable domains secreted fromEscherichia coli, Nature, 1989, 341: 544.PubMedCrossRefGoogle Scholar
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    Vaughan, T. J., Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library,Nature Biotechnology, 1996, 14: 309.PubMedCrossRefGoogle Scholar
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    Cai, X. H., Garen, A., Anti-melanoma antibodies from melanoma patients immunized with genetically modified autologous tumor cells: selection of pecific antibodies from single-chain Fv fusion phage libraries,Proc. Natl. Acad. Sci. USA, 1995, 92: 6537.PubMedCrossRefGoogle Scholar
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    Griffith, A. D., Williams, S. C., Hartley, O.et al., Isolation of high affinity human antibodies directly from large synthetic repertoires,The EMBO Journal, 1994, 13: 3245.Google Scholar
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    Low, N. M., Mimicking somatic hypermutation: Affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain,J. Mol. Biol., 1996, 260: 359.PubMedCrossRefGoogle Scholar
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    Marks, J. D., By passing immunisation: high affinity human antibodies by chain shuffling, Bio/technology, 1992, 10: 779.PubMedCrossRefGoogle Scholar

Copyright information

© Science in China Press 1997

Authors and Affiliations

  • Xiyun Yan
    • 1
  • Bo Tian
    • 1
  1. 1.Institute of MicrobiologyChinese Academy of SciencesBeijingChina

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