An ABA-binding protein with nucleic acid-binding property
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Maize cDNA expression library was screened using the antibodies against ABA-binding proteins (ABBP). From 200 000 separated phage plaques, only one cDNA clone coding for a polypeptide was obtained. It shows 60%–65% homology to the genes of nucleic acid-binding proteins in mammalia. However, about 120 cDNA clones coding maize 17s rRNA were unexpectedly obtained. In view of this, the ABBP purified by ABA affinity chromatography and the antibodies derived from the purified ABBP were examined. It is found that rRNA is indeed present in the ABBP. In addition, the obtained antibodies could recognize RNA as well as ABBP. It is suggested that the ABBP have nucleic acid-binding property and can form a complex with rRNA.
KeywordsABA-binding proteins ABBP rRNA anti-ABBP sera cDNA expression library immunological screening
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