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Science in China Series C: Life Sciences

, Volume 41, Issue 2, pp 209–216 | Cite as

An ABA-binding protein with nucleic acid-binding property

  • Zhifu Zheng
  • Zhenhua Jin
  • Xie Zhou
  • Kai Xia
  • Cheng Ma
Article

Abstract

Maize cDNA expression library was screened using the antibodies against ABA-binding proteins (ABBP). From 200 000 separated phage plaques, only one cDNA clone coding for a polypeptide was obtained. It shows 60%–65% homology to the genes of nucleic acid-binding proteins in mammalia. However, about 120 cDNA clones coding maize 17s rRNA were unexpectedly obtained. In view of this, the ABBP purified by ABA affinity chromatography and the antibodies derived from the purified ABBP were examined. It is found that rRNA is indeed present in the ABBP. In addition, the obtained antibodies could recognize RNA as well as ABBP. It is suggested that the ABBP have nucleic acid-binding property and can form a complex with rRNA.

Keywords

ABA-binding proteins ABBP rRNA anti-ABBP sera cDNA expression library immunological screening 

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Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Zhifu Zheng
    • 1
  • Zhenhua Jin
    • 2
  • Xie Zhou
    • 1
  • Kai Xia
    • 1
  • Cheng Ma
    • 2
  1. 1.Agronomy DepartmentNanjing Agricultural UniversityNanjingChina
  2. 2.Institute of Development BiologyChinese Academy of SciencesBeijingChina

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