Injecting skin fibroblasts into muscles to express human clotting factor IX cDNA
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Primary rabbit skin fibroblasts (RSF) containing lacZ reporter gene or human clotting factor IX (hFIX) cDNA could long-termly exist and be expressed after being implanted in rabbit muscles. Among them, the beta-glactosidase expression lasted at least 3 months, and hFIX expression lasted 1 month. These results will be helpful to organically combine the advantages of both skin fibroblasts and muscle, and provide a new way for somatic gene therapy of hemophilia B and other genetic diseases.
Keywordsmuscle injection of fibroblast expression of human clotting factor IX
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