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Science in China Series C: Life Sciences

, Volume 41, Issue 2, pp 146–150 | Cite as

Injecting skin fibroblasts into muscles to express human clotting factor IX cDNA

  • Hongwei Wang
  • Xiaoqing Yang
  • Yongna Xing
  • Daru Lu
  • Xinfang Qiu
  • Jinglun Xue
Article

Abstract

Primary rabbit skin fibroblasts (RSF) containing lacZ reporter gene or human clotting factor IX (hFIX) cDNA could long-termly exist and be expressed after being implanted in rabbit muscles. Among them, the beta-glactosidase expression lasted at least 3 months, and hFIX expression lasted 1 month. These results will be helpful to organically combine the advantages of both skin fibroblasts and muscle, and provide a new way for somatic gene therapy of hemophilia B and other genetic diseases.

Keywords

muscle injection of fibroblast expression of human clotting factor IX 

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Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Hongwei Wang
    • 1
  • Xiaoqing Yang
    • 1
  • Yongna Xing
    • 1
  • Daru Lu
    • 1
  • Xinfang Qiu
    • 1
  • Jinglun Xue
    • 1
  1. 1.Institute of GeneticsFudan UniversityShanghaiChina

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