Biologia Plantarum

, Volume 29, Issue 6, pp 437–444 | Cite as

Formation of adventitious buds in needle primordia isolated from embryonic shoots of adult picea abies

  • P. Krogstrup
Original Papers


Needle primordia were isolated from embryonic shoot expiants of 88 year oldPicea abies (Norway spruce) in order to develop a method for micropropagation using adult mother trees. The isolation of needle primordia in combination with frequent subculture, stimulated the formation of peripheral meristematic tissue in expiants cultured on a modified Murashige & Skoog medium supplemented with cytokinin. Transfer of isolated needle primordia with peripheral meristematic tissue to medium without growth regulators, and with a reduced ratio of NH+4/ NO−3, resulted in the formation of adventitious buds.


Picea Abies Abscission Zone Subculture Frequency Intact Epidermis Embryonic Shoot 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aitken, J., Horgan, K. J., Thorpe, T. A.: Influence of explant selection on the shoot forming capacity of juvenile tissue of Radiata pine. - Can. J. Forest Res.11: 112–117, 1981.CrossRefGoogle Scholar
  2. Biondi, S., Thorpe, T. A.: Clonal propagation of forest tree species. - In: Rao, A. N. (ed.): Proc. COSTED Symp. on Tissue Culture of Economically Important Plants, 1981. Pp. 197 to 204. Singapore 1982.Google Scholar
  3. Bonga, J. M.: Vegetative propagation in relation to juvenility, maturity and rejuvenation. - In: Bonga, J. M., Durzan, D. J. (ed.): Tissue Culture in Forestry. Pp. 387–413. M. Nijhoff/ Dr W. Junk Publ., The Hague—Boston—London 1982.Google Scholar
  4. Chlyah, H.: Correlations in organogenesis. - In: Novák, F. J., Havel, L., Doležel, J. (ed.): Proc. Int. Symp. Olomouc, Czechoslovakia. Pp. 57–67. Czeohosl. Acad. Sci., Praha 1984.Google Scholar
  5. David, A., David, H., Mateille, T.:In vitro adventitious budding ofPinus pinaster cotyledons and needles. - Physiol. Plant.56: 102–107, 1982.CrossRefGoogle Scholar
  6. Dormling, I., Kellerstam, H.: Rooting and rejuvenation in propagating old Norway spruce cuttings. - In: Research Notes 32. Pp. 65 - 72. Uppsala 1981.Google Scholar
  7. Franclet, A.: Rajeunissement et micropropagation des ligneux. [Rejuvenation and micropropagation of woody plants]. - In Colloque Int. sur la Culture “in vitro” des Essences Forestières. Pp. 55–65. AFOCEL, Nangis 1981.Google Scholar
  8. Green, P. B.: Organogenesis - a biophysical view. - Annu. Rev. Plant Physiol.31: 51–82, 1980.CrossRefGoogle Scholar
  9. Haissig, B. E.: Influences of auxins and auxin synergists on adventitious root primordium initiation and development. - N. Zeal. J. Forest Soi.4: 311–323, 1974.Google Scholar
  10. Hearne, D. A.: Preliminary report on a technique which provides a “maturity factor” for trees grown in tissue culture. -Proc. int. Plant Prop. Soc.32: 109–114, 1982.Google Scholar
  11. Jansson, E., Bornman, C. H.: Morphogenesis in dormant embryonic shoots ofPicea abies: Influence of the crown and cold treatment. - Physiol. Plant.59: 1–8, 1983.CrossRefGoogle Scholar
  12. Krogstrup, P.: Embryo-like structures from cotyledons and ripe embryos of Norway spruce(Picea abies). - Can. J. Forest Res.16: 664–668, 1986.CrossRefGoogle Scholar
  13. Lintilhac, P. M., Vesecky, T. B.: Mechanical stress and cell wall orientation in plants. II The application of controlled directional stress to growing plants. - Amer. J. Bot.68: 1222–1230, 1981.CrossRefGoogle Scholar
  14. Mott, R. L., Cobdts, J. M., Larson, A. M.: Nitrogen and growth regulator effect on shoot and root growth of soybeanin vitro. - In: Henke, R. (ed): Tissue Culture in Forestry and Agriculture. Pp. 336–337. Plenum Press, New York 1985.Google Scholar
  15. Mubashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant.15: 473–497, 1962.CrossRefGoogle Scholar
  16. Osborne, D. J.: The ethylene regulation of cell growth in specific tissues of plants - In: Wareing, P.F. (ed.): Plant Growth Substances. Pp. 279–290. Academic Press, London 1982.Google Scholar
  17. Sriskandarajah, S., Mullins, M. G., Nair, Y.: Induction of adventitious rooting in difficult to propagate cultivars of apple. - Plant Sci. Lett.24: 1–9, 1982.CrossRefGoogle Scholar
  18. von Abnold, S.: Importance of genotype in the potential forin vitro adventitious bud production ofPicea abies. -Forest Sci.30: 314–318, 1984.Google Scholar
  19. von Arnold, S., Eriksson, T.: Induction of adventitious buds on buds of Norway spruce(Picea abies) grownin vitro. - Physiol. Plant.45: 29–34, 1979.CrossRefGoogle Scholar
  20. Zajaczkowski, S., Wodzicki, T. J., Bruinsma, J.: A possible mechanism for whole plant morphogenesis. -Physiol. Plant.57: 306–310, 1983.CrossRefGoogle Scholar

Copyright information

© Academia 1987

Authors and Affiliations

  • P. Krogstrup
    • 1
  1. 1.Botanical GardenUniversity of CopenhagenCopenhagen KDenmark

Personalised recommendations