Biologia Plantarum

, Volume 29, Issue 6, pp 437–444 | Cite as

Formation of adventitious buds in needle primordia isolated from embryonic shoots of adult picea abies

  • P. Krogstrup
Original Papers


Needle primordia were isolated from embryonic shoot expiants of 88 year oldPicea abies (Norway spruce) in order to develop a method for micropropagation using adult mother trees. The isolation of needle primordia in combination with frequent subculture, stimulated the formation of peripheral meristematic tissue in expiants cultured on a modified Murashige & Skoog medium supplemented with cytokinin. Transfer of isolated needle primordia with peripheral meristematic tissue to medium without growth regulators, and with a reduced ratio of NH+4/ NO−3, resulted in the formation of adventitious buds.


Picea Abies Abscission Zone Subculture Frequency Intact Epidermis Embryonic Shoot 
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Copyright information

© Academia 1987

Authors and Affiliations

  • P. Krogstrup
    • 1
  1. 1.Botanical GardenUniversity of CopenhagenCopenhagen KDenmark

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