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Formation of adventitious buds in needle primordia isolated from embryonic shoots of adult picea abies

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Biologia Plantarum

Abstract

Needle primordia were isolated from embryonic shoot expiants of 88 year oldPicea abies (Norway spruce) in order to develop a method for micropropagation using adult mother trees. The isolation of needle primordia in combination with frequent subculture, stimulated the formation of peripheral meristematic tissue in expiants cultured on a modified Murashige & Skoog medium supplemented with cytokinin. Transfer of isolated needle primordia with peripheral meristematic tissue to medium without growth regulators, and with a reduced ratio of NH+4/ NO−3, resulted in the formation of adventitious buds.

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  1. Botanical Garden, University of Copenhagen, Oester Farimagsgade 2B, DK-1353, Copenhagen K, Denmark

    P. Krogstrup

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  1. P. Krogstrup
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Krogstrup, P. Formation of adventitious buds in needle primordia isolated from embryonic shoots of adult picea abies. Biol Plant 29, 437–444 (1987). https://doi.org/10.1007/BF02882217

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  • DOI: https://doi.org/10.1007/BF02882217

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