Effect of DNA binding protein Ssh12 from hyperthermophilic archaeonSulfolobus shibatae on DNA supercoiling

  • Huiqiang Lou
  • Li Huang
  • Viet Q. Mai


An 11.5-ku DNA binding protein, designated as Ssh12, was purified from the hyperthermophilic archaeonSulfolobus shibatae by column chromatography in SP Sepharose, DNA cellulose and phosphocellulose. Ssh12 accounts for about 4 % of the total cellular protein. The protein is capable of binding to both negatively supercoiled and relaxed DNAs. Nick closure analysis revealed that Ssh12 constrains negative supercoils upon binding to DNA. While the ability of the protein to constrain supercoils is weak at 22°C, it is enhanced substantially at temperatures higher than 37°C. Both the cellular content and supercoil-constraining ability of Ssh12 suggest that the protein may play an important role in the organization and stabilization of the chromosome ofS. shibatae.


hyperthermophilic archaea Sulfolobus shibatae DNA binding protein supercoiling 


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Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Huiqiang Lou
    • 1
  • Li Huang
    • 1
  • Viet Q. Mai
    • 2
  1. 1.State Key Laboratory of Microbial Resources, Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  2. 2.Pomona CollegeUSA

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