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Structural feature of sorghum chloroplastpsbA gene and regulation effects of its 5′-noncoding region

  • Naihu Wu
  • Xiaohua Fang
  • Xiaomei Shi
  • Xiaowu Zhang
  • Li Zhou
  • Meijuan Huang
Article

Abstract

Comparative analysis reveals remarkahle homology between the sequences of bothpsbA gene nucleotides and the inferred amino acids of sorghum, a C4 plant, and those of rice, a C3 plant. The 5′-noncoding region of sorghumpsbA gene contains the conservative promoter elements, “—35” element and “—10” element, like the prokaryote and the promoter element, TATA box, like the eukaryote. As compared with that of the rice, an extra sequence of 7 bp is found in the leader sequence of the mRNA in the former. Using anin vitro system, it has been demonstrated that protein factor exists in sorghum chloroplast protein extract which specifically hinds to the 5′-noncoding region ofpsbA gene. Measurement of the expression of luciferase shows a 2–5 time greater reaction of the expression plasmids pALqs which contain leader region of sorghumpsbA gene than that of the expression plasmids pALqr which contain leader region of ricepsbA gene inE. coli.

Keywords

sorghum chloroplast psbA gene promoter structure 5′-noncoding region transcription regulation 

References

  1. 1.
    Nanba, O., Satoch, K., Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559,Proc. Natl. Acad. USA, 1987, 84: 101.CrossRefGoogle Scholar
  2. 2.
    Wu, N. H., Cote, C. J., Wu, R., Structure of the chloroplastpsbA gene encoding the Qβ protein fromOryza sativa L.,Develop. Genet., 1987, 8: 339.CrossRefGoogle Scholar
  3. 3.
    Link, G., Photocontrol of plastid gene expression,Plant Cell and Environment, 1988, 11: 329.CrossRefGoogle Scholar
  4. 4.
    Gilmartin, P. M., Sarokin, L., Memelink, J. et al., Molecular light switches for plant genes,The Plant Cell, 1990, 2: 369.PubMedCrossRefGoogle Scholar
  5. 5.
    Hughes, J. E., Link, G., Photoregulation ofpsbA transcript levels in mustard cotyledons, inMolecular Biology of Photosynthesis (eds. Govindjee et al.), the Netherlands: Kluwer Academic Publishers, 1988, 423–429.Google Scholar
  6. 6.
    Danon, A., Translational regulation in the chloroplast,Plant Physiol., 1997, 115: 1293.PubMedCrossRefGoogle Scholar
  7. 7.
    Zhong, Z. P., Wu, N. H., Expressional regulationin vitro ofpsbA mRNA 3′ untranslated region in rice and sorghum,Science in China, Ser. C, 1997, 40(4): 406.CrossRefGoogle Scholar
  8. 8.
    Conard, S. G., Radosevich, S. R., Ecological fitness ofSenecio vulgaris andAmaranthus retroflexus biotypes susceptible and resistant to atrazine,J. Appl. Ecol., 1979, 16: 171.CrossRefGoogle Scholar
  9. 9.
    Holt, J. S., Stemler, A. J., Radosevich, S. R., Differential light-responses of photosynthesis by triazine-resistant and triazine-susceptibleSenecio vulgaris biotypes,Plant Physiol., 1981, 67: 744.PubMedCrossRefGoogle Scholar
  10. 10.
    Wu, N. H., Studies on ricepsbA gene,Genetic Manipulation in Crops (ed. Li Xianghui) (in Chinese), Beijing: Higher Education Press, 1994, 96–190.Google Scholar
  11. 11.
    Himse, T., Sugiura, M., Cis-acting elements and trans-acting factors for accurate translation of chloroplastpsbA mRNAs: development of anin vitro translation system from tobacco chloroplasts,The EMBO Journal, 1996, 15(7): 1687.Google Scholar
  12. 12.
    Wu, N. H., Shi, X. M., Isolation and characterization of the chloroplastpsbA gene from sorghum (Sorghum. vulgare L.),Chinese J. Bot., 1991, 3: 81.Google Scholar
  13. 13.
    Shi, X. M., Wu, N. H. et al., Analysis of 5′-noncoding region of sorghum light-regulatedpsbA gene,Chinese Science Bulletin, 1994, 39(14): 1224.Google Scholar
  14. 14.
    Wu, N. H., Fan, T. S., Ren, D. L., Mutational effect of the “-35” element of sorghumpsbA gene promoter,Chinese Science Bulletin, in press.Google Scholar
  15. 15.
    Huang Yao, Wu Naihu, Characterization of 5′-noncoding region of millet chloroplastpsbA gene,Developmental and Reproductive Biology, 1995, 4(2): 17.Google Scholar

Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Naihu Wu
    • 1
  • Xiaohua Fang
    • 1
    • 2
  • Xiaomei Shi
    • 1
  • Xiaowu Zhang
    • 1
  • Li Zhou
    • 1
    • 2
  • Meijuan Huang
    • 2
  1. 1.Institute of Developmental BiologyChinese Academy of SciencesBeijingChina
  2. 2.School of Life SciencesPeking UniversityBeijingChina

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