Science in China Series C: Life Sciences

, Volume 42, Issue 1, pp 109–112 | Cite as

Gene therapy for rat renal anemia with implantation of erythropoietin-transgenic myoblasts

  • Liu Yongxue 
  • Handong Wei
  • Zuze Wu
  • Fuchu He


To investigate whether an erythropoietin (EPO) gene-based therapy could serve as an alternative to the repeated injection of rhEPO in treatment to renal anemia, the genetically modified myoblasts of rats, named Myo/ EPO, were implanted through intramuscular injection to model rats with renal anemia. The hemoglobin (Hb) and hematocrit (HCT) of the rats increased from (92.5 ±3.0) g/L and 0.29±0.04 to the peak values of (103.8 ±5.0) g/L and 0. 32 ±0. 04 respectively 14 d after implantation, and sustained the pre-implantation level for 90 d. Otherwise, the control rats implanted with Myo/X, which carried the parent retroviral vector, gradually became severe in anemia. The PCR detection for hEPO cDNA in the rat muscle adjacent to injection sites indicated that the Myo/EPO cells survived for a long period in the muscle of rats. The results primarily demonstrate that myoblast gene transfer of EPO is effective for the treatment of rat renal anemia.


rat renal anemia erythropoietin gene therapy 


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  1. 1.
    Krantz, S. B., Erythropoietin,Blood, 1991, 77: 41.Google Scholar
  2. 2.
    Naffakh, N., Henri, A., Villeval, J. L. et al., Sustained delivery of erythropoietin in mice by genetically modified skin fibroblasts,PNAS, 1995, 92:3194.PubMedCrossRefGoogle Scholar
  3. 3.
    Osborne, W. R. A., Ramesh, N., Lau, S. et al., Gene therapy for long-term expression of erythropoietin in rats,PNAS, 1995, 92:8055.PubMedCrossRefGoogle Scholar
  4. 4.
    Setogushi, Y., Danel, C., Crystal, R. G., Stimulation of erythopoiesis byin vivo gene therapy: physiologic consequences of transfer of the human erythropoietin gene to experimental animals using an denovirus vector,Blood, 1994, 84:2946.Google Scholar
  5. 5.
    Descamps, V., Blumenfeld, N., Villeval, J. L. et al., Erythropoietin gene transfer and expression in adult normal mice: use of an adenovirus vector,Human Gene Therapy, 1994, 5:979.PubMedCrossRefGoogle Scholar
  6. 6.
    Liu, Y. X., Wei, H. D., Wu, Z. Z. et al., Characteristics and identification of a rat model induced with adenine diet,Chinese Journal of Nephrology (in Chinese), 1998, 14(1):53.Google Scholar
  7. 7.
    Qu, C. K., Wei, H. D., He, F. C. et al., hEPO cDNA transfer mediated by retroviral vector and its expression in the primary culture of rats,Chinese Science Bulletin (in Chinese), 1995, 40:2192.Google Scholar
  8. 8.
    Blau, H. M., Dhawan, J., Pavlath, G. K., Myoblasts in pattern formation and gene therapy,TIG, 1993, 9:269.PubMedGoogle Scholar
  9. 9.
    Harnamori, Y., Samal, B., Tian, J. et al., Persistent erythropoiesis by myoblast transfer of erythropoietin cDNA,Human Gene Therapy, 1994, 5:1349.CrossRefGoogle Scholar
  10. 10.
    Egrie, J. C., Sickland, T. W., Lane, J. et al., Characterization and biological effects of recombinant human erythropoietin,Immunobiology, 1989, 172:213.Google Scholar
  11. 11.
    Cynshi, O., Satoh, K., Higuchi, M. et al., Effects of recombinant erythropoietin on anemic W/Wv and SI/SId mice,British Journal Hematology, 1990, 75:319.CrossRefGoogle Scholar

Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Liu Yongxue 
    • 1
  • Handong Wei
    • 1
  • Zuze Wu
    • 1
  • Fuchu He
    • 1
  1. 1.Beijing Institute of Radiation MedicineBeijingChina

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