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Gene therapy for rat renal anemia with implantation of erythropoietin-transgenic myoblasts

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Abstract

To investigate whether an erythropoietin (EPO) gene-based therapy could serve as an alternative to the repeated injection of rhEPO in treatment to renal anemia, the genetically modified myoblasts of rats, named Myo/ EPO, were implanted through intramuscular injection to model rats with renal anemia. The hemoglobin (Hb) and hematocrit (HCT) of the rats increased from (92.5 ±3.0) g/L and 0.29±0.04 to the peak values of (103.8 ±5.0) g/L and 0. 32 ±0. 04 respectively 14 d after implantation, and sustained the pre-implantation level for 90 d. Otherwise, the control rats implanted with Myo/X, which carried the parent retroviral vector, gradually became severe in anemia. The PCR detection for hEPO cDNA in the rat muscle adjacent to injection sites indicated that the Myo/EPO cells survived for a long period in the muscle of rats. The results primarily demonstrate that myoblast gene transfer of EPO is effective for the treatment of rat renal anemia.

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Authors and Affiliations

  1. Beijing Institute of Radiation Medicine, 100850, Beijing, China

    Liu Yongxue, Handong Wei, Zuze Wu & Fuchu He

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  1. Liu Yongxue
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  2. Handong Wei
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  3. Zuze Wu
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  4. Fuchu He
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Project supported by the National Natural Science Foundation of China (Grant No. 39470321).

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Liu, Y., Wei, H., Wu, Z. et al. Gene therapy for rat renal anemia with implantation of erythropoietin-transgenic myoblasts. Sci. China Ser. C.-Life Sci. 42, 109–112 (1999). https://doi.org/10.1007/BF02881756

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  • DOI: https://doi.org/10.1007/BF02881756

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