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Science in China Series C: Life Sciences

, Volume 42, Issue 6, pp 591–598 | Cite as

Study of thrombopoietin for gene therapy of thrombocytopenia

  • Song Chong
  • Daru Lu
  • Changben Li
  • Xinfang Qiu
  • Jinglun Xue
Article
  • 12 Downloads

Abstract

Thrombopoietin (TPO) is likely to he a potent, specific and reliable medication in the treatment of thrombocytopenia. A TPO-highly-expressed plasmid pcDNA3-TPO was constructed and a primary study was made on the expression of TPO cDNAin vitro and gene transfer study for thrombocytopeniain vivo. rhTPO showed complete and stable bioactivity by a series of indicators. High expression of TPO was detected in plasma from healthy mice or thrombocytopenia mice model receiving direct intramuscular injection of pcDNA3-TPO. And the platelet level of healthy mice peaked to 1.9-fold of baseline. Mice with CTX-induced thrombocytopenia achieved profound nadirs, acceleration of recovery, even 1.8–2.0-fold supranormal levels of peripheral platelet counts. The results offered experimental support for clinical application of gene therapy for thrombocytopenia via direct intramuscular injection of TPO cDNA.

Keywords

thrombopoietin (TPO) gene transfer thrombocytopenia intramuscular injection 

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Copyright information

© Science in China Press 1999

Authors and Affiliations

  • Song Chong
    • 1
  • Daru Lu
    • 1
  • Changben Li
    • 1
  • Xinfang Qiu
    • 1
  • Jinglun Xue
    • 1
  1. 1.Institute of GeneticsFudan UniversityShanghaiChina

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