Biologia Plantarum

, Volume 24, Issue 6, pp 415–422 | Cite as

The effect ofN-nitroso-N-ethylurea on mutagenesis ofCercospora beticola SACC

  • Dorota Brilová
Original Papers


Lethal and mutagenic effects ofN-nitroso-N-ethylurea (NEU) on the parasitic fungusCercospora beticola Sacc. was investigated. Mutation frequency increased and the number of surviving individuals (conidia) simultaneously decreased with increasing mutagen concentration and the period of its application.

Treatment ofC. beticola conidia with NEU induced 14 morphological mutants characterized by changes in pigmentation of air mycelium and substrate, excretion of the pigment into the cultivation medium and colonies morphology. A considerable proportion of morphological mutants lost their sporulation ability bothin vitro on the sporulation medium andin vivo on host leaves and became non-pathogenic. The other morphological mutants (21.4%) were pathogenic on a sensitive sugar beet cultivar Dobrovická A and on other three resistant cultivars (Maribo 1, 2, 3).

A revertant with increased pathogenity arose from the light non-pathogenic mutant. During the remonosporic isolation of the pathogenic mutants isolates were obtained characterized by a phenotype which originated during the mutagenic process.


Wild Strain Morphological Mutant Sugar Beet Cultivar Colour Sector Biochemical Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abbondandolo, A., Simi, S.: Mosaicism and lethal sectoring in G1 cells ofSchizosaccharomyces pombae. -Mut. Res.12: 143–150, 1971.Google Scholar
  2. Bajaj, B. S., Chatrath, M. S., Vasudeva, R. S.: Mutation inColletotrichum falcatum Went. the causal organism of rod sugarcancer. IV. Morphological and biochemical mutants induced by ionizing radiation. - Indian Phytopathol.4: 296–303, 1964.Google Scholar
  3. Boone, D. M., Keitt, G. W.:Venturia inaequalis (Cke) Wint. VIII. Inheritance of color mutant characters. -Amer. J. Bot.43: 226–233, 1956.CrossRefGoogle Scholar
  4. Boone, D. M., Stauffer, J. F., Stahmann, M. A., Keitt, G. W.:Venturia inaequalis (Cke) Wint. VII. Induction of mutants for studies on genetics, nutrition, and pathogenicity. - Amer. J. Bot.43: 199–204, 1956.CrossRefGoogle Scholar
  5. Campbell, R., Larner, R. W., Madelin, M. F.: Notes on an albino mutant ofAlternaria brassicola. - Mycologia60: 1122–1125, 1968.PubMedCrossRefGoogle Scholar
  6. Canova, A.: [Research on Biology and Epidemiology ofCercospora beticola Sacc.] In Ital. - Agraria, Roma 1959.Google Scholar
  7. Dutta, S. K., Garber, E. D.: Genetics of phytopathogenie fungi. III. An attempt to demonstrate the parasexual cycle inColletotrichum lagenarium. - Bot. Gaz.122: 118–121, 1960.CrossRefGoogle Scholar
  8. Eckardt, F., Haynes, R. H.: Induction of pure and sectored mutant clones in excision-proficient and deficient strains of yeast. - Mut, Res.43: 327–338, 1977.Google Scholar
  9. Frandsen, N. O.: Untersuchungen überCercospora beticola III. Morphologie dos Pilzes. - Zucker8: 513–515, 1955.Google Scholar
  10. Guglielminetti, R.: The role of lethal sectoring in the origin of complete mutations inSchizosaccharomyces pombae. - Mut. Res.5: 225–229, 1968.Google Scholar
  11. Hannan, M. A., Duck, P., Nasim, A.: UV-induced lethal sectoring and pure mutant clones in yeast. -Mut. Res.36: 171–176, 1976.Google Scholar
  12. Hastie, A. C.: Genetic recombination in the hop-wilt fungusVerticillium albo-atrum. - J. gen. Microbiol.27: 373–382, 1962.PubMedGoogle Scholar
  13. James, A. P., Nasim, A., McCullough, R. S.: The nature of replicating instability in yeast. -Mut. Res.15: 125–133, 1972.Google Scholar
  14. James, A. P., Werner, M. M.: Radiation induced genetic mosaicism and lethal sectoring in yeast. - Mut. Res.3: 477–485, 1966.Google Scholar
  15. Keitt, G. W., Boone, D. M.: Induction and inheritance of mutant characters inVenturia inaequalis in relation to its pathogenicity. - Phytopathology44: 362–370, 1954.Google Scholar
  16. Levitin, M. M., Zakharov, I. A.: [The lethal and mutagenie effect of UV rays in the cause of browning and stem break of flax.] In Russ. - Genetika9: 65 - 70, 1967.Google Scholar
  17. Nasim, A.: The induction of replicating instabilities by mutagens inSchizosaccharomyces pombae. - Mut. Res.4: 753–763, 1967.Google Scholar
  18. Nasim, A., Grant, C.: Genetic analysis of replicating instabilities in yeast. - Mut. Res.17: 185 to 190, 1973.Google Scholar
  19. Novotel’nova, N. S., Pystina, K. A.: [Induced variability of the fungus causing the verticillium wilt of cotton plants.] In Russ. - Mikol. Fitopatol.4: 290–300, 1968.Google Scholar
  20. Plotho, O.: Weitere Untersuchungen zur Zytologie und Morphologie derCercospora beticola. - Zucker5: 379–383, 1952.Google Scholar
  21. Remneva, Z. I., Ivanyuk, V. G.: [Interspecific inhomogeneity inMacrosporium solani Ell. et Mart. the cause of early blight of potato.] In Russ. - Mikol. Fitopatol.3: 202–208, 1968.Google Scholar
  22. Tinline, R. D., Stauffer, J. F., Dickson, J. G.:Cochlyobolus sativus. III. Effect of ultraviolet radiation. - Can. J. Bot.38: 275–282, 1960.CrossRefGoogle Scholar
  23. Tuveson, R. W., Garber, E. D.: Genetics of phytopathogenic fungi. II. The parasexual cycle inFusarium oxysporum f. pisi. - Bot. Gaz.121: 74–80, 1959.CrossRefGoogle Scholar

Copyright information

© Academia 1982

Authors and Affiliations

  • Dorota Brilová
    • 1
  1. 1.Institute of Experimental Phytopathology and EnthomologySlovak Academy of SciencesIvanka pri DunajiCzechoslovakia

Personalised recommendations